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Human VF(Visfatin) ELISA Kit

Citations(16)Uniprot : P43490

Cat.No.:E-EL-H1763
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.93	2.10	7.95	0.96	2.20	7.16
Standard deviation	0.06	0.09	0.33	0.05	0.09	0.26
CV (%)	6.45	4.29	4.15	5.21	4.09	3.63

Recovery

The recovery of Human VF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-104	95
EDTA plasma (n=8)	93-105	100
Cell culture media (n=8)	87-101	93

Linearity

Samples were spiked with high concentrations of Human VF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P43490
Synonyms	Nampt, NAmPRTase, PBEF1, pre-B-cell colony-enhancing factor 1, Nicotinamide Phosphoribosyltransferase
Research Area	Cancer, Cardiovascular, Metabolism, Immunology, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Oncotarget (2016) IF: 5.008
Adipocytes secreted leptin is a pro-tumor factor for survival of multiple myeloma under chemotherapy

DOI: 10.18632/oncotarget.13342

Sample: plasma
JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 5.25
Increased uncoupling protein 1 levels are associated with attention deficit and hyperactivity disorder in medication-free children

DOI: 10.1016/j.jpsychires.2022.10.047

PMID: 36327766
Sample: serum
Journal of Clinical Medicine (2021) IF: 4.242
Relationship between Adipokines and Cardiovascular Ultrasound Parameters in Metabolic-Dysfunction-Associated Fatty Liver Disease

DOI: 10.3390/jcm10215194

PMID: 34768714
Sample: serum
Scientific Reports (2021) IF: 4.38
Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3

PMID: 33859244
Sample: Plasma
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

DOI: 10.1016/j.diabres.2019.05.023

PMID: 31150725
Sample: Serum
World Journal of Diabetes (2020) IF: 3.247
Relationship between diabetic polyneuropathy, serum visfatin, and oxidative stress biomarkers

DOI: 10.4239/wjd.v11.i7.309

PMID: 32843933
Sample: Serum
ARCHIVES OF DERMATOLOGICAL RESEARCH (2018) IF: 2.148
Visfatin and insulin levels and cigarette smoking are independent risk factors for hidradenitis suppurativa: a case–control study

DOI: 10.1007/s00403-018-1867-z

Sample: Serum
Obesity Research & Clinical Practice (2022) IF: 2.288
The effect of omentectomy on the blood levels of adipokines in obese patients with endometrial cancer

DOI: 10.1016/j.orcp.2022.06.002

PMID: 35701296
Sample: serum
Dermatologic Therapy (2020) IF: 2.327
Correlation of metabolic syndrome with serum omentin‐1 and visfatin levels and disease severity in psoriasis and psoriatic arthritis

DOI: 10.1111/dth.14378

Sample: Serum
Lipids in Health and Disease (2019) IF: 2.651
Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus

DOI: 10.1186/s12944-019-1169-2

PMID: 31836012
Sample: Serum
JOURNAL OF DIABETES AND ITS COMPLICATIONS (2021) IF: 2.852
Effects of increased physical activity and/or weight loss diet on serum myokine and adipokine levels in overweight adults with impaired glucose metabolism

DOI: 10.1016/j.jdiacomp.2021.107892

PMID: 33685795
Sample: Serum
MEDICINE (2021) IF: 1.889
Association of serum vaspin, apelin, and visfatin levels and stroke risk in a Chinese case-control study

DOI: 10.1097/MD.0000000000025184

PMID: 33761698
Sample: Serum
MEDICAL SCIENCE MONITOR (2019) IF: 1.98
Triglyceride-to-High-Density Lipoprotein Cholesterol Ratio and Systemic Inflammation in Patients with Idiopathic Pulmonary Arterial Hypertension

DOI: 10.12659/MSM.912766

PMID: 30683836
Sample: Serum
Clinical Diabetology (2020)
Serum magnesium and visfatin levels in newly diagnosed non-obese type 2 diabetes without insulin resistance: a cross-sectional study

DOI: 10.5603/DK.2020.0063

Sample: serum
Clinical Hypertension (2020)
Serum adipocytokines and adiposity as predictive indices of preeclampsia

DOI: 10.1186/s40885-020-00152-0

PMID: 33014422
Sample: Serum,Urine
Turkish Journal Of Medical Sciences (2021) IF: 0.973
The relationship of serum visfatin levels with clinical parameters, flow-mediated dilation, and carotid intima-media thickness in patients with ankylosing spondylitis

DOI: 10.3906/sag-2012-351

Sample: serum

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