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Human VF(Visfatin) ELISA Kit

  • Cat.No.:E-EL-H1763

  • Reactivity: Human

To Purchase E-EL-H1763

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.93 2.10 7.95 0.96 2.20 7.16
Standard deviation 0.06 0.09 0.33 0.05 0.09 0.26
CV (%) 6.45 4.29 4.15 5.21 4.09 3.63

Recovery

The recovery of Human VF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 95
EDTA plasma (n=8) 93-105 100
Cell culture media (n=8) 87-101 93

Linearity

Samples were spiked with high concentrations of Human VF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P43490
SynonymsNampt, NAmPRTase, PBEF1, pre-B-cell colony-enhancing factor 1, Nicotinamide Phosphoribosyltransferase

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Diabetes research and clinical practice (2019) IF: 4.234
    Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

    DOI: 10.1016/j.diabres.2019.05.023

    PMID: 31150725

    Sample: Serum
  2. World Journal of Diabetes (2020) IF: 3.247
    Relationship between diabetic polyneuropathy, serum visfatin, and oxidative stress biomarkers

    DOI: 10.4239/wjd.v11.i7.309

    PMID: 32843933

    Sample: Serum
  3. Dermatologic Therapy (2020) IF: 2.327
    Correlation of metabolic syndrome with serum omentin-1 and visfatin levels and disease severity in psoriasis and psoriatic arthritis

    DOI: 10.1111/dth.14378

    PMID: 33029930

    Sample: Serum
  4. Clinical Hypertension (2020) IF: 2.719
    Serum adipocytokines and adiposity as predictive indices of preeclampsia

    DOI: 10.1186/s40885-020-00152-0

    PMID: 33014422

    Sample: Serum,Urine
  5. Lipids in health and disease (2019) IF: 2.906
    Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus

    DOI: 10.1186/s12944-019-1169-2

    PMID: 31836012

    Sample: Serum
  6. Archives of dermatological research (2018) IF: 2.148
    Visfatin and insulin levels and cigarette smoking are independent risk factors for hidradenitis suppurativa: a case-control study.

    DOI: 10.1007/s00403-018-1867-z

    PMID: 30293125

    Sample: Serum
  7. Reumatologia (2020) IF: 1.81
    Serum Chemerin and Visfatin Levels and Their Ratio as Possible Diagnostic Parameters of Rheumatoid Arthritis

    DOI: 10.5114/reum.2020.95359

    PMID: 32476678

    Sample: Serum
  8. Indian journal of dermatology (2019) IF: 1.338
    Assessment of Metabolic Profile and Ischemia-modified Albumin Level in Patients With Alopecia Areata: A Case-Control Study

    DOI: 10.4103/ijd.IJD_238_18

    PMID: 30745629

    Sample: Serum
  9. Medical science monitor: international medical journal of experimental and clinical research (2019)
    Triglyceride-to-High-Density Lipoprotein Cholesterol Ratio and Systemic Inflammation in Patients with Idiopathic Pulmonary Arterial Hypertension

    DOI: 10.12659/MSM.912766

    PMID: 30683836

    Sample: Serum
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