Micro-Sample

Mini Sample Human EGF (Epidermal Growth Factor) ELISA Kit (E-MSEL-H0017)

Name: Mini Sample Human EGF (Epidermal Growth Factor) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0017
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Mini Sample Human EGF (Epidermal Growth Factor) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	2.447	2.511	2.479	2.403
250	1.747	1.807	1.777	1.701
125	0.922	0.886	0.904	0.828
62.5	0.472	0.486	0.479	0.403
31.25	276.000	0.268	0.272	0.196
15.63	0.182	0.186	0.184	0.108
7.81	0.165	0.167	0.166	0.090
0	0.078	0.074	0.076	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.700	51.470	191.480	27.960	48.620	195.610
Standard deviation	1.560	2.320	11.700	2.280	3.920	16.140
CV（%）	6.080	4.510	6.110	8.140	8.070	8.250

Recovery

The recovery of Human EGF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	94
EDTA plasma （n=8）	95-111	102
Cell culture media （n=8）	88-103	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human EGF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-97	97-114	96-111
1:2	Average	90	105	103
1:4	Range	83-95	96-110	95-110
1:4	Average	89	103	102
1:8	Range	81-94	100-111	94-111
1:8	Average	86	105	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	87.44-109.14
Sample 2（n=16）	93.43-100.51

Background

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.