Micro-Sample

Mini Sample Human FGF23 ( Fibroblast Growth Factor 23) ELISA Kit (E-MSEL-H0012)

Name: Mini Sample Human FGF23 ( Fibroblast Growth Factor 23) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0012
Price: 150.0 USD
Availability: InStock

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Mini Sample Human FGF23 ( Fibroblast Growth Factor 23) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes FGF23 in samples.No significant cross-reactivity or interference between FGF23 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.206	2.106	2.156	2.076
2000	1.475	1.519	1.497	1.417
1000	0.789	0.809	0.799	0.719
500	0.484	0.512	0.498	0.418
250	0.258	0.274	0.266	0.186
125	0.176	0.164	0.170	0.090
62.5	0.149	0.141	0.145	0.065
0	0.083	0.077	0.080	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	207.280	392.530	1585.500	223.430	397.060	1608.160
Standard deviation	11.500	24.020	95.920	17.760	33.670	104.850
CV（%）	5.550	6.120	6.050	7.950	8.480	6.520

Recovery

The recovery of Human FGF23 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	98
EDTA plasma （n=8）	93-107	98
Cell culture media （n=8）	91-104	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FGF23 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-106	86-101	99-113
1:2	Average	97	93	105
1:4	Range	88-99	86-97	97-112
1:4	Average	93	92	104
1:8	Range	94-107	88-104	100-111
1:8	Average	99	95	106

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	92.40-98.61
Sample 2（n=16）	109.86-113.94

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF23 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF23 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FGF23 . You can calculate the concentration of FGF23 in the samples by comparing the OD of the samples to the standard curve.

Background

Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.