Micro-Sample
Mini Sample Human GAL3 (Galectin 3) ELISA Kit (E-MSEL-H0032)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 0.19 ng/mL |
| Detection Range | 0.31-20 ng/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes GAL3 in samples.No significant cross-reactivity or interference between GAL3 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Human GAL3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human GAL3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GAL3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GAL3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 32 nm. The OD value is proportional to the concentration of GAL3 . You can calculate the concentration of GAL3 in the samples by comparing the OD of the samples to the standard curve.
Background
Galectin-3 is a soluble β-galactoside binding protein, widely distributed in the heart, kidney, liver, lung and intestinal tissues and organs. Galectin-3 has a variety of biological functions, it can bind to a variety of ligands on the cell surface and extracellular matrix, and plays an important role in a series of physiological and pathological processes, including apoptosis, adhesion, proliferation, migration, inflammatory response, immune response, and fibrosis processes. In atherosclerosis, cardiac remodeling and myocardial fibrosis, Galectin 3 is abnormally overexpressed and secreted into the blood circulation. Galectin 3 can also enhance platelet aggregation, adhesion and retraction, and promote thrombosis. It is widely believed that it is involved in the growth and spread of cancer cells, can induce the secretion of protease in intestinal cancer cells, enhance the aggressiveness of cancer cells, and reduce the integrity of the epithelial mesenchyma between cancer cells. It is up-regulated in liver cancer and involved in the occurrence and development of liver cancer. It is also related to the occurrence and development of many diseases such as organ fibrosis, chronic inflammation and scar formation.
| Gene Alias | LGALS3 |
| Gene ID | 3958 |
| Uniport ID | P17931 |
| Protein Alias | LGALS3 |
| Research Area | Cellbiology , Neuroscience |
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