Micro-Sample
Mini Sample Human IL-3 (Interleukin-3) ELISA Kit (E-MSEL-H0028)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 46.88 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes IL-3 in samples.No significant cross-reactivity or interference between IL-3 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Human IL-3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human IL-3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 28 nm. The OD value is proportional to the concentration of IL-3 . You can calculate the concentration of IL-3 in the samples by comparing the OD of the samples to the standard curve.
Background
Interleukin-3 (lL-3) is a hemopoietic growth factor involved in the survival,proliferation,and differentiation of multipotent hemopoietic cells. In addition to granulocyte-macrophage colony-stimulating factor (GMCSF) and IL-5,IL-3 is the most potent growth factor for basophils. On mature basophils,these three cytokines act through specific receptors mediating adhesion,migration,and releasability. Mast cells expresses high amounts of IL-3. In the absence of antigen,rapid release of large amount of IL-3 in an autocrine dependent manner is responsible for mast cell survival. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases,including asthma,atherosclerosis,and neurodegenerative disorders,such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes,neutrophils,and macrophages from bone marrow cultures. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.
| Gene Alias | IL3 |
| Gene ID | 3562 |
| Uniport ID | P08700 |
| Protein Alias | IL3 |
| Research Area | CellBiology , SignalTransduction , StemCells |
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