Micro-Sample

Mini Sample Human IL-8 (Interleukin 8) ELISA Kit (E-MSEL-H0043)

Name: Mini Sample Human IL-8 (Interleukin 8) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0043
Price: 150.0 USD
Availability: InStock

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Mini Sample Human IL-8 (Interleukin 8) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.503	2.395	2.449	2.367
1000	1.763	1.769	1.766	1.684
500	1.011	1.017	1.014	0.932
250	0.591	0.573	0.582	0.500
125	0.302	0.304	0.303	0.221
62.5	0.193	0.185	0.189	0.107
31.25	0.135	0.145	0.140	0.058
0	0.080	0.084	0.082	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.070	306.740	825.060	88.240	327.610	839.950
Standard deviation	5.310	17.640	50.580	7.310	20.280	56.700
CV（%）	5.650	5.750	6.130	8.280	6.190	6.750

Recovery

The recovery of Human IL-8 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-108	101
EDTA plasma （n=8）	88-104	95
Cell culture media （n=8）	92-106	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IL-8 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-101	98-112	94-109
1:2	Average	93	106	101
1:4	Range	84-98	95-110	94-107
1:4	Average	90	102	100
1:8	Range	83-97	98-114	91-108
1:8	Average	89	104	98

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	101.60-109.54
Sample 2（n=16）	85.48-110.40

Background

Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells, and endothelial cells.In humans, it is encoded by the IL-8 gene.IL-8 can effectively promote angiogenesis and play an important role in the pathogenesis of bronchiolitis. IL-8 can be induced by the recruitment and degranulation of neutrophils.It can act as an autocrine growth factor in colon cancer cell lines by regulating the inflammatory response. High levels of IL-8 reduce schizophrenia.Positive reaction to antipsychotic drugs in patients with schizophrenia. In addition, IL-8 has been implicated in the pathogenesis of obesity and cystic fibrosi.