Micro-Sample
Mini Sample Human MMP-9 (Matrix metalloproteinase-9) ELISA Kit (E-MSEL-H0026)
Add to cart
For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 23.44 pg/mL |
| Detection Range | 39-2500 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes MMP-9 in samples.No significant cross-reactivity or interference between MMP-9 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Recovery |
The recovery of Human MMP-9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human MMP-9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
|
|||||||||||||||||||||||||||||||||||||||||||||
| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
|
|||||||||||||||||||||||||||||||||||||||||||||
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MMP-9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MMP-9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 26 nm. The OD value is proportional to the concentration of MMP-9 . You can calculate the concentration of MMP-9 in the samples by comparing the OD of the samples to the standard curve.
Background
The full name of MMP-9 in Chinese is matrix metalloproteinase 9. The MMP-9 gene is located on chromosome 20q11.1-13.1, 26-27kpp, with 13 exons and 9 introns. It belongs to the matrix metalloproteinase (MMP) family. The main function of MMP-9 is to degrade and reshape the dynamic balance of extracellular matrix. The homology of MMP-9 mRNA in mammals is about 80%. MMP-9 can decompose structural complexes such as ECM and basement membrane in the respiratory tract and lungs, thus participating in the reconstruction of the respiratory tract and lungs. It can also regulate the activity of other proteases and cytokines. It can degrade alpha 1 antitrypsin, protect neutrophil elastase activity, enhance collagen cell and MMP-13 sol-gel activity in collagen colloids. MMP-9 can also break down a 62 amino acid peptide from interleukin-8 (CXCL8/CL8), increasing its chemotactic activity towards neutrophils by 10 times, but it also inhibits other neutrophil chemotactic factors. In addition, MMP-9 binds to CD44 to release stored TGF - β 1, and MMP-9 can participate in angiogenesis by releasing vascular endothelial growth factor (VEGF).
| Gene Alias | MMP9 |
| Gene ID | 4318 |
| Uniport ID | P14780 |
| Protein Alias | MMP9 |
| Research Area | Angiogenesis , CellBiology , StemCells |
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Videos
Resources
Contact with Us
Toll-free: 1-888-852-8623
Tel: 1-832-243-6086
Add:
14780 Memorial Drive, Suite 105,
Houston, Texas, 77079, USA
Houston, Texas, 77079, USA
