Micro-Sample

Mini Sample Human NGAL ( Neutrophil Gelatinase Associated Lipocalin ) ELISA Kit (E-MSEL-H0007)

Name: Mini Sample Human NGAL ( Neutrophil Gelatinase Associated Lipocalin ) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0007
Price: 150.0 USD
Availability: InStock

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Mini Sample Human NGAL ( Neutrophil Gelatinase Associated Lipocalin ) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes NGAL in samples.No significant cross-reactivity or interference between NGAL and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.440	2.434	2.434	2.345
1000	1.618	1.562	1.590	1.473
500	0.990	0.972	0.972	0.883
250	0.546	0.550	0.548	0.461
125	0.302	0.312	0.307	0.223
62.5	0.186	0.190	0.186	0.101
31.25	0.120	0.121	0.121	0.032
0	0.088	0.089	0.089	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	107.370	220.380	971.060	101.680	198.560	1009.380
Standard deviation	5.520	15.250	54.960	8.100	15.570	73.790
CV（%）	5.140	6.920	5.660	7.970	7.840	7.310

Recovery

The recovery of Human NGAL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	96
EDTA plasma （n=8）	87-100	92
Cell culture media （n=8）	92-107	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human NGAL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-99	93-107	89-104
1:2	Average	92	99	97
1:4	Range	84-96	91-102	93-107
1:4	Average	90	96	99
1:8	Range	87-102	90-104	87-100
1:8	Average	94	95	93

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	95.68-104.51
Sample 2（n=16）	90.82-112.50

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NGAL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NGAL , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of NGAL . You can calculate the concentration of NGAL in the samples by comparing the OD of the samples to the standard curve.

Background

NGAL, also known as lipocalin 2, LCN2, and siderocalin, is a small molecule composed of 178 amino acids. It belongs to the lipocalin superfamily of 20 structurally related secreted proteins. NGAL occurs predominantly in a monomeric form, with a small percentage occurring as a dimer or trimer. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. It is a critical component of innate immunity because its ability to bind iron-siderophore complexes limits bacterial growth. Human NGAL is expressed at very low levels in human tissues, including kidney, trachea, lungs, stomach, and colon, and its expression increases greatly in the presence of inflammation and injured epithelia. In addition to its role in innate immunity, it is a promising biomarker for acute kidney injury that is beginning to be used in clinical practice in addition to research studies.