Micro-Sample

Mini Sample Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit (E-MSEL-M0011)

Name: Mini Sample Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0011
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse CCL12/MCP-5 (Monocyte Chemotactic Protein 5) ELISA Kit - 1

Size	Price	Qty
96T	$ 625.00	- +
48T	$ 500.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	3.97 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CCL12/MCP-5 in samples.No significant cross-reactivity or interference between Mouse CCL12/MCP-5 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	2.239	2.203	2.221	2.145
250	1.550	1.578	1.564	1.488
125	0.980	0.984	0.982	0.906
62.5	0.492	0.498	0.495	0.419
31.25	0.305	0.319	0.312	0.236
15.63	0.186	0.182	0.184	0.108
7.81	0.129	0.133	0.131	0.055
0	0.075	0.077	0.076	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.700	71.500	243.750	28.540	64.370	265.590
Standard deviation	1.920	4.190	14.500	2.200	5.520	19.680
CV（%）	6.940	5.860	5.950	7.720	8.580	7.410

Recovery

The recovery of Mouse CCL12 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-103	96
EDTA plasma （n=8）	88-101	95
Cell culture media （n=8）	85-100	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CCL12 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-113	98-112	97-110
1:2	Average	103	106	103
1:4	Range	98-114	101-117	93-106
1:4	Average	104	107	99
1:8	Range	97-111	96-112	94-107
1:8	Average	102	103	99

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	96.79-102.14
Sample 2（n=16）	95.30-106.67

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CCL12/MCP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CCL12/MCP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CCL12/MCP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CCL12/MCP-5. You can calculate the concentration of Mouse CCL12/MCP-5 in the samples by comparing the OD of the samples to the standard curve.

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