Mini Sample Mouse ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit (E-MSEL-M0095)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Eotaxin 1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Eotaxin 1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Eotaxin 1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Eotaxin 1 . You can calculate the concentration of Eotaxin 1 in the samples by comparing the OD of the samples to the standard curve.

CCL11, also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. The full length of CCL11 is 97 amino acids (a.a.) with a 23 a.a. signal peptide, which is cleaved to generate a 74 a.a. mature protein. The molecular mass of CCL11 is about 10 kD, and its gene is located on the q arm of chromosome 17. CCL11 expression has been found in normal tissues such as smooth muscle and small intestine. Endothelial cells, smooth muscle cells, epithelial cells, alveolar macrophages, and eosinophils are known to produce CCL11. Mouse CCL11 shares a 61% and 96% amino acid sequence identity to both human and rat, respectively.