Micro-Sample

Mini Sample Mouse IgA ( Immunoglobulin A ) ELISA Kit (E-MSEL-M0079)

Name: Mini Sample Mouse IgA ( Immunoglobulin A ) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0079
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Mini Sample Mouse IgA ( Immunoglobulin A ) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	3.75 ng/mL
Detection Range	6.25-400 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes IgA in samples.No significant cross-reactivity or interference between IgA and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
400	2.475	2.413	2.444	2.353
200	1.763	1.747	1.755	1.664
100	1.031	1.069	1.050	0.959
50	0.551	0.521	0.536	0.445
25	0.309	0.313	0.311	0.220
12.5	0.200	0.200	0.200	0.109
6.25	0.150	0.148	0.149	0.058
0	0.089	0.093	0.091	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	18.430	40.050	188.230	17.020	43.300	181.060
Standard deviation	1.180	2.480	10.010	1.090	2.810	13.980
CV（%）	6.400	6.180	5.320	6.400	6.490	7.720

Recovery

The recovery of Mouse IgA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-101	93
EDTA plasma （n=8）	90-103	96
Cell culture media （n=8）	90-105	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IgA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	86-99	96-112
1:2	Average	91	92	103
1:4	Range	82-94	87-102	92-105
1:4	Average	87	94	99
1:8	Range	86-100	83-95	93-109
1:8	Average	92	89	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	90.18-109.03
Sample 2（n=16）	92.68-95.39

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgA . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgA , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IgA . You can calculate the concentration of IgA in the samples by comparing the OD of the samples to the standard curve.

Background

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

Research Area

Immunology