Micro-Sample

Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit (E-MSEL-M0116)

Name: Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0116
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse PF4 (Platelet Factor 4) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes PF4 in samples.No significant cross-reactivity or interference between PF4 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.236	2.352	2.294	2.200
500	1.372	1.382	1.377	1.283
250	0.796	0.792	0.794	0.700
125	0.501	0.501	0.501	0.407
62.5	0.284	0.288	0.286	0.192
31.25	0.181	0.189	0.185	0.091
15.63	0.139	0.129	0.134	0.040
0	0.097	0.091	0.094	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.080	83.440	433.550	50.400	75.650	464.570
Standard deviation	2.610	3.610	24.110	3.400	5.690	41.720
CV（%）	5.220	4.330	5.560	6.740	7.520	8.980

Recovery

The recovery of Mouse PF4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-96	91
EDTA plasma （n=8）	95-109	100
Cell culture media （n=8）	88-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse PF4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-101	94-109	93-111
1:2	Average	92	100	101
1:4	Range	83-96	89-105	92-105
1:4	Average	90	96	98
1:8	Range	85-97	91-106	94-107
1:8	Average	90	98	99

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	92.04-108.17
Sample 2（n=16）	92.04-95.88

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PF4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PF4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 41 nm. The OD value is proportional to the concentration of PF4 . You can calculate the concentration of PF4 in the samples by comparing the OD of the samples to the standard curve.

Background

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence. Chemotactic for neutrophils and monocytes via CCR1. Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis. In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition. Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity.