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Mouse Aβ1-42(Amyloid Beta 1-42) ELISA Kit

  • Cat.No.:E-EL-M3010

  • Reactivity: Mouse

To Purchase E-EL-M3010

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.13-200 pg/mL
Sensitivity 1.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse Aβ1-42 in samples. No significant cross-reactivity or interference between Mouse Aβ1-42 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-42 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Aβ1-42. You can calculate the concentration of Mouse Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 9.10 21.66 98.11 8.24 21.52 85.62
Standard deviation 0.53 1.07 4.93 0.43 1.06 3.64
CV (%) 5.82 4.94 5.02 5.22 4.93 4.25


The recovery of Mouse Aβ1-42 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-99 95
EDTA plasma (n=8) 85-94 90
Cell culture media (n=8) 97-106 100


Samples were spiked with high concentrations of Mouse Aβ1-42 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P12023
SynonymsAβ42,Amyloid Beta 42

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular Neurobiology (2016) IF: 6.19
    Cnga2 Knockout Mice Display Alzheimer's-Like Behavior Abnormities And Pathological Changes.

    DOI: 10.1007/s12035-015-9421-x

    PMID: 26377105

    Sample: Tissue homogenate
  2. Aging (Albany NY) (2020) IF: 4.831
    Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice

    DOI: 10.18632/aging.103445

    PMID: 32652518

    Sample: Tissue homogenate
  3. Experimental Cell Research (2020) IF: 3.383
    Study of mitophagy and ATP-related metabolomics based on β-amyloid levels in Alzheimer's disease

    DOI: 10.1016/j.yexcr.2020.112266

    PMID: 32905804

    Sample: Cell lysate
  4. International Journal of Molecular Sciences (2017) IF: 3.226
    Spirulina Maxima Extract Ameliorates Learning And Memory Impairments Via Inhibiting GSK-3β Phosphorylation Induced by Intracerebroventricular Injection of Amyloid-β 1-42 In Mice.

    DOI: 10.3390/ijms18112401

    PMID: 29137190

    Sample: Tissue homogenate
  5. Neurochemical research (2019) IF: 2.772
    Resveratrol Abrogates Hypoxia-Induced Up-Regulation of Exosomal Amyloid-β Partially by Inhibiting CD147

    DOI: 10.1007/s11064-019-02742-3

    PMID: 30771155

    Sample: Tissue homogenate,Cell culture medium
  6. Plos One (2017) IF: 2.806
    A Novel Monoclonal Antibody Against The N-Terminus Of Aβ1-42 Reduces Plaques And Improves Cognition In A Mouse Model Of Alzheimer’S Disease.

    DOI: 10.1371/journal.pone.0180076

    PMID: 28662102

    Sample: Serum,Tissue homogenate
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