Click here to view cell apoptosis assay products
×

Mouse Aβ1-42(Amyloid Beta 1-42) ELISA Kit

  • Cat.No.:E-EL-M3010

  • Reactivity: Mouse

To Purchase E-EL-M3010

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.13-200 pg/mL
Sensitivity 1.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse Aβ1-42 in samples. No significant cross-reactivity or interference between Mouse Aβ1-42 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-42 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Aβ1-42. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Aβ1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Aβ1-42, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Aβ1-42. You can calculate the concentration of Mouse Aβ1-42 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 9.10 21.66 98.11 8.24 21.52 85.62
Standard deviation 0.53 1.07 4.93 0.43 1.06 3.64
CV (%) 5.82 4.94 5.02 5.22 4.93 4.25

Recovery

The recovery of Mouse Aβ1-42 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-99 95
EDTA plasma (n=8) 85-94 90
Cell culture media (n=8) 97-106 100

Linearity

Samples were spiked with high concentrations of Mouse Aβ1-42 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P12023
SynonymsAβ42,Amyloid Beta 42
Research AreaCancer, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Autophagy (2019) IF: 11.059
    Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model

    DOI: 10.1080/15548627.2019.1596488

    PMID: 30898012

    Sample: Tissue homogenate,Cell culture supernatant,Cell ly
  2. PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
    Interleukin-17 (IL-17) triggers systemic inflammation, peripheral vascular dysfunction, and related prothrombotic state in a mouse model of Alzheimer's disease

    DOI: 10.1016/j.phrs.2022.106595

    Sample: plasma
  3. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
    Dendrobium officinale polysaccharide attenuates cognitive impairment in circadian rhythm disruption mice model by modulating gut microbiota

    DOI: 10.1016/j.ijbiomac.2022.07.090

    PMID: 35853505

    Sample: hippocampus
  4. Frontiers in Immunology (2022) IF: 8.786
    Bifidobacterium breve intervention combined with environmental enrichment alleviates cognitive impairment by regulating the gut microbiota and microbial metabolites in Alzheimer's disease mice.

    DOI: 10.3389/fimmu.2022.1013664

    Sample: Hippocampal tissue
  5. CNS Neuroscience & Therapeutics (2023) IF: 7.035
    Apicidin attenuates memory deficits by reducing the Aβ load in APP/PS1 mice

    DOI: 10.1111/cns.14102

    Sample: Brain tissue
  6. Neurotherapeutics (2022) IF: 7.62
    Chk1 Inhibition Ameliorates Alzheimer’s Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling

    DOI: 10.1007/s13311-022-01204-z

    Sample: brain tissue,hippocampus
  7. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Chronic MC-LR exposure promoted Aβ and p-tau accumulation via regulating Akt/GSK-3β signal pathway

    DOI: 10.1016/j.scitotenv.2021.148732

    PMID: 34323745

    Sample: HT-22 cell
  8. Neurotherapeutics (2020) IF: 6.035
    Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer’s Disease Pathogenesis in Cell and Animal Models

    DOI: 10.1007/s13311-020-00841-6

    Sample: N2a/APP cell
  9. BIOFACTORS (2021) IF: 6.113
    Berberine ameliorates neuronal AD-like change via activating Pi3k/PGCε pathway

    DOI: 10.1002/biof.1725

    PMID: 33740285

    Sample: Cell culture supernatant
  10. TRAFFIC (2022) IF: 6.144
    AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice

    DOI: 10.1111/tra.12874

    Sample: Hippocampus tissue
  11. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    Dysregulated APP expression and α-secretase processing of APP is involved in manganese-induced cognitive impairment

    DOI: 10.1016/j.ecoenv.2021.112365

    PMID: 34058678

    Sample: Plasma
  12. PHYTOTHERAPY RESEARCH (2022) IF: 6.388
    3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy

    DOI: 10.1002/ptr.7619

    Sample: brain tissue
  13. Nutrients (2022) IF: 6.706
    Moringa Oleifera Alleviates Aβ Burden and Improves Synaptic Plasticity and Cognitive Impairments in APP/PS1 Mice

    DOI: 10.3390/nu14204284

    Sample: brain tissue
  14. Nutrients (2022) IF: 6.706
    Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes

    DOI: 10.3390/nu14153179

    Sample: cerebral tissue,BV2 cell
  15. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953
    Effects of heparan sulfate from porcine mucosa on Aβ1–42-induced neurotoxicity in vitro and in vivo

    DOI: 10.1016/j.ijbiomac.2022.03.079

    Sample: peripheral plasma,brain
  16. MOLECULAR NEUROBIOLOGY (2015) IF: 5.137
    Cnga2 Knockout Mice Display Alzheimer’s-Like Behavior Abnormities and Pathological Changes

    DOI: 10.1007/s12035-015-9421-x

    Sample: Tissue homogenate
  17. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020) IF: 5.162
    Yeast β-glucan alleviates cognitive deficit by regulating gut microbiota and metabolites in Aβ1–42-induced AD-like mice

    DOI: 10.1016/j.ijbiomac.2020.05.180

    Sample: hippocampus tissue
  18. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Anti-depressive-like and cognitive impairment alleviation effects of Gastrodia elata Blume water extract is related to gut microbiome remodeling in ApoE−/− mice exposed to unpredictable chronic mild stress

    DOI: 10.1016/j.jep.2022.115872

    Sample: serum
  19. Journal of Functional Foods (2022) IF: 5.223
    Camellia oil improves Aβ25-35-induced memory impairment by regulating the composition of the gut microbiota and lipid metabolism in mice

    DOI: 10.1016/j.jff.2022.105214

    Sample: brain
  20. MOLECULAR NUTRITION & FOOD RESEARCH (2020) IF: 5.309
    Quercetin‐3‐O‐Glucuronide Alleviates Cognitive Deficit and Toxicity in Aβ1‐42‐Induced AD‐Like Mice and SH‐SY5Y Cells

    DOI: 10.1002/mnfr.202000660

    Sample: Tissue homogenate
  21. PHYTOMEDICINE (2022) IF: 5.34
    Corallodiscus flabellata B. L. Burtt extract and isonuomioside A ameliorate Aβ25−35-induced brain injury by inhibiting apoptosis, oxidative stress, and autophagy via the NMDAR2B/CamK Ⅱ/PKG pathway

    DOI: 10.1016/j.phymed.2022.154114

    Sample: Brain tissue
  22. Aging-US (2021) IF: 5.682
    Platelets transport β-amyloid from the peripheral blood into the brain by destroying the blood-brain barrier to accelerate the process of Alzheimer's disease in mouse models

    DOI: 10.18632/aging.202662

    PMID: 33668038

    Sample: Serum
  23. Frontiers in Aging Neuroscience (2022) IF: 5.75
    Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis

    DOI: 10.3389/fnagi.2021.809433

    Sample: brain tissue
  24. Frontiers in Aging Neuroscience (2022) IF: 5.75
    Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice

    DOI: 10.3389/fnagi.2021.766410

    Sample: hippocampus tissue
  25. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2017) IF: 5.924
    Spirulina maxima Extract Ameliorates Learning and Memory Impairments via Inhibiting GSK-3β Phosphorylation Induced by Intracerebroventricular Injection of Amyloid-β 1–42 in Mice

    DOI: 10.3390/ijms18112401

    Sample: Tissue homogenate
  26. BRAIN RESEARCH BULLETIN (2021) IF: 4.079
    MicroRNA-455–5p/CPEB1 pathway mediates Aβ-related learning and memory deficits in a mouse model of Alzheimer’s disease

    DOI: 10.1016/j.brainresbull.2021.10.008

    Sample: hippocampi tissue
  27. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2022) IF: 4.114
    Thymidine and 2′-deoxyuridine reduce microglial activation and improve oxidative stress damage by modulating glycolytic metabolism on the Aβ25-35-induced brain injury

    DOI: 10.1016/j.abb.2022.109377

    Sample: Brain tissue
  28. Food & Function (2020) IF: 4.171
    High-altitude Tibetan fermented milk ameliorated cognitive dysfunction by modified gut microbiota in Alzheimer's disease transgenic mice

    DOI: 10.1039/C9FO03007G

    PMID: 32458851

    Sample: Serum
  29. ACS Chemical Neuroscience (2018) IF: 4.211
    Ganglioside Hp-s1 Analogue Inhibits Amyloidogenic Toxicity in Alzheimer’s Disease Model Cells

    DOI: 10.1021/acschemneuro.8b00406

    Sample: N2a cells
  30. NEUROTOXICOLOGY (2021) IF: 4.294
    Pathological methamphetamine exposure triggers the accumulation of neuropathic protein amyloid-β by inhibiting UCHL1

    DOI: 10.1016/j.neuro.2021.06.004

    PMID: 34175320

    Sample: hippocampus
  31. NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
    The role of LRP1 in Aβ efflux transport across the blood-brain barrier and cognitive dysfunction in diabetes mellitus

    DOI: 10.1016/j.neuint.2022.105417

    PMID: 36067928

    Sample: brain tissue
  32. MOLECULES (2022) IF: 4.412
    Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25–35-Induced Mice by Inhibiting the NLRP3 Inflammasome

    DOI: 10.3390/molecules27082488

    Sample: brain
  33. ACS Chemical Neuroscience (2022) IF: 4.418
    Effect of the MAGL/FAAH Dual Inhibitor JZL-195 on Streptozotocin-Induced Alzheimer’s Disease-like Sporadic Dementia in Mice with an Emphasis on Aβ, HSP-70, Neuroinflammation, and Oxidative Stress

    DOI: 10.1021/acschemneuro.1c00699

    Sample: hippocampus
  34. ACS Chemical Neuroscience (2020) IF: 4.418
    Biophysical, Biochemical, and Behavioral Implications of ApoE3 Conjugated Donepezil Nanomedicine in a Aβ1–42 Induced Alzheimer’s Disease Rat Model

    DOI: 10.1021/acschemneuro.0c00430

    Sample: hippocampus
  35. JOURNAL OF ALZHEIMERS DISEASE (2021) IF: 4.472
    NPD1 Enhances Autophagy and Reduces Hyperphosphorylated Tau and Amyloid-β 42 by Inhibiting GSK3β Activation in N2a/APP695swe Cells

    DOI: 10.3233/JAD-210729

    Sample: N2a cell
  36. MOLECULAR NEUROBIOLOGY (2019) IF: 4.586
    Expression of AHI1 Rescues Amyloidogenic Pathology in Alzheimer’s Disease Model Cells

    DOI: 10.1007/s12035-019-1587-1

    Sample: N2a cells
  37. Aging-US (2020) IF: 4.831
    Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice

    DOI: 10.18632/aging.103445

    PMID: 32652518

    Sample: Tissue homogenate
  38. ACTA NEUROPSYCHIATRICA (2020) IF: 3
    Anti-HIV drugs promote β-amyloid deposition and impair learning and memory in BALB/c mice

    DOI: 10.1017/neu.2020.19

    Sample: Hippocampal tissue
  39. NEUROTOXICOLOGY (2018) IF: 3.076
    Ameliorative effect of alendronate against intracerebroventricular streptozotocin induced alteration in neurobehavioral, neuroinflammation and biochemical parameters with emphasis on Aβ and BACE-1

    DOI: 10.1016/j.neuro.2018.11.012

    PMID: 30481507

    Sample: Tissue homogenate
  40. EXPERIMENTAL GERONTOLOGY (2019) IF: 3.08
    Plasma amyloid beta level changes in aged mice with cognitive dysfunction following sevoflurane exposure

    DOI: 10.1016/j.exger.2019.110737

    Sample: plasma
  41. BRAIN RESEARCH (2022) IF: 3.252
    Adenosine ameliorated Aβ25−35-induced brain injury through the inhibition of apoptosis and oxidative stress via an ERα pathway

    DOI: 10.1016/j.brainres.2022.147944

    Sample: cerebral cortex tissue
  42. BRAIN RESEARCH BULLETIN (2020) IF: 3.37
    Neuroprotective role of alendronate against APP processing and neuroinflammation in mice fed a high fat diet

    DOI: 10.1016/j.brainresbull.2020.04.010

    PMID: 32389800

    Sample: Tissue homogenate
  43. JOURNAL OF DRUG TARGETING (2020) IF: 3.38
    Development, optimisation and evaluation of chitosan nanoparticles of alendronate against Alzheimer’s disease in intracerebroventricular streptozotocin model for brain delivery

    DOI: 10.1080/1061186X.2020.1817041

    Sample: Tissue homogenate
  44. EXPERIMENTAL CELL RESEARCH (2020) IF: 3.383
    Study of mitophagy and ATP-related metabolomics based on β-amyloid levels in Alzheimer's disease

    DOI: 10.1016/j.yexcr.2020.112266

    PMID: 32905804

    Sample: Cell lysate
  45. PSYCHOPHARMACOLOGY (2019) IF: 3.424
    Chronic stress impairs the aquaporin-4-mediated glymphatic transport through glucocorticoid signaling

    DOI: 10.1007/s00213-018-5147-6

    PMID: 30607477

    Sample: Tissue homogenate
  46. JOURNAL OF APPLIED TOXICOLOGY (2021) IF: 3.446
    Alendronate reduces the cognitive and neurological disturbances induced by combined doses of d-galactose and aluminum chloride in mice

    DOI: 10.1002/jat.4160

    PMID: 33694194

    Sample: Cell lysate
  47. NEUROSCIENCE LETTERS (2019) IF: 2.173
    Mouse induced pluripotent stem cells-derived Alzheimer’s disease cerebral organoid culture and neural differentiation disorders

    DOI: 10.1016/j.neulet.2019.134433

    Sample: iPSCs cells
  48. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.292
    Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer’s disease

    DOI: 10.1080/00207454.2020.1733564

    Sample: brain
  49. NEUROCHEMICAL RESEARCH (2019) IF: 2.782
    TREM2 Attenuates Aβ1-42-Mediated Neuroinflammation in BV-2 Cells by Downregulating TLR Signaling

    DOI: 10.1007/s11064-019-02817-1

    PMID: 31134514

    Sample: Cell culture supernatant
  50. NEUROCHEMICAL RESEARCH (2019) IF: 2.782
    Resveratrol Abrogates Hypoxia-Induced Up-Regulation of Exosomal Amyloid-β Partially by Inhibiting CD147

    DOI: 10.1007/s11064-019-02742-3

    PMID: 30771155

    Sample: Tissue homogenate,Cell culture medium
  51. PLoS One (2017) IF: 2.806
    A novel monoclonal antibody against the N-terminus of Aβ1-42 reduces plaques and improves cognition in a mouse model of Alzheimer’s disease

    DOI: 10.1371/journal.pone.0180076

    Sample: Serum,Tissue homogenate
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha: