Current Position:Home>>Kits>>ELISA Kits>> Mouse Aβ1-42(Amyloid Beta 1-42) ELISA Kit
Cat.No.:E-EL-M3010
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 3.13-200 pg/mL |
Sensitivity | 1.88 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse Aβ1-42 in samples. No significant cross-reactivity or interference between Mouse Aβ1-42 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-42 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Aβ1-42 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 9.10 | 21.66 | 98.11 | 8.24 | 21.52 | 85.62 |
Standard deviation | 0.53 | 1.07 | 4.93 | 0.43 | 1.06 | 3.64 |
CV (%) | 5.82 | 4.94 | 5.02 | 5.22 | 4.93 | 4.25 |
The recovery of Mouse Aβ1-42 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 89-99 | 95 |
EDTA plasma (n=8) | 85-94 | 90 |
Cell culture media (n=8) | 97-106 | 100 |
Samples were spiked with high concentrations of Mouse Aβ1-42 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P12023
|
Synonyms | Aβ42,Amyloid Beta 42 |
Research Area | Cancer, Neuroscience |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 30898012 |
Sample: Tissue homogenate,Cell culture supernatant,Cell ly |
Sample: plasma |
PMID: 35853505 |
Sample: hippocampus |
Sample: Hippocampal tissue |
DOI: 10.1111/cns.14102 |
|
Sample: Brain tissue |
Sample: brain tissue,hippocampus |
PMID: 34323745 |
Sample: HT-22 cell |
Sample: N2a/APP cell |
DOI: 10.1002/biof.1725 |
|
PMID: 33740285 |
Sample: Cell culture supernatant |
DOI: 10.1111/tra.12874 |
|
Sample: Hippocampus tissue |
PMID: 34058678 |
Sample: Plasma |
DOI: 10.1002/ptr.7619 |
|
Sample: brain tissue |
DOI: 10.3390/nu14204284 |
|
Sample: brain tissue |
DOI: 10.3390/nu14153179 |
|
Sample: cerebral tissue,BV2 cell |
Sample: peripheral plasma,brain |
Sample: Tissue homogenate |
Sample: hippocampus tissue |
Sample: serum |
Sample: brain |
Sample: Tissue homogenate |
Sample: Brain tissue |
PMID: 33668038 |
Sample: Serum |
Sample: brain tissue |
Sample: hippocampus tissue |
DOI: 10.3390/ijms18112401 |
|
Sample: Tissue homogenate |
Sample: hippocampi tissue |
Sample: Brain tissue |
DOI: 10.1039/C9FO03007G |
|
PMID: 32458851 |
Sample: Serum |
Sample: N2a cells |
PMID: 34175320 |
Sample: hippocampus |
PMID: 36067928 |
Sample: brain tissue |
Sample: brain |
Sample: hippocampus |
Sample: hippocampus |
DOI: 10.3233/JAD-210729 |
|
Sample: N2a cell |
Sample: N2a cells |
PMID: 32652518 |
Sample: Tissue homogenate |
DOI: 10.1017/neu.2020.19 |
|
Sample: Hippocampal tissue |
PMID: 30481507 |
Sample: Tissue homogenate |
Sample: plasma |
Sample: cerebral cortex tissue |
PMID: 32389800 |
Sample: Tissue homogenate |
Sample: Tissue homogenate |
PMID: 32905804 |
Sample: Cell lysate |
PMID: 30607477 |
Sample: Tissue homogenate |
DOI: 10.1002/jat.4160 |
|
PMID: 33694194 |
Sample: Cell lysate |
Sample: iPSCs cells |
Sample: brain |
PMID: 31134514 |
Sample: Cell culture supernatant |
PMID: 30771155 |
Sample: Tissue homogenate,Cell culture medium |
Sample: Serum,Tissue homogenate |