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Mouse ACTH(Adrenocorticotropic Hormone) ELISA Kit

  • Cat.No.:E-EL-M0079

  • Reactivity: Mouse

To Purchase E-EL-M0079

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACTH. During the reaction, ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ACTH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ACTH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.20 119.30 479.60 47.00 128.20 475.90
Standard deviation 3.10 6.20 24.00 3.10 6.00 26.20
CV (%) 5.94 5.20 5.00 6.60 4.68 5.51


The recovery of ACTH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma(n=8) 88-104 95
Cell culture media(n=8) 89-102 96


Samples were spiked with high concentrations of Mouse ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCorticotropin, POMC, Adrenocorticotropin
Research AreaCancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    Uncarialins J‐M from Uncaria rhynchophylla and their anti‐depression mechanism in unpredictable chronic mild stress‐induced mice via activating 5‐HT1A receptor

    DOI: 10.1002/cjoc.202000652

    Sample: lung
  2. FASEB JOURNAL (2021) IF: 5.192
    High-fat diet-induced increases in glucocorticoids contribute to the development of non-alcoholic fatty liver disease in mice

    DOI: 10.1096/fj.202101570R

    PMID: 34959259

    Sample: plasma
  3. Journal of Functional Foods (2022) IF: 5.223
    Juglanin ameliorates depression-like behavior in chronic unpredictable mild stress-induced mice by improving AMPK signaling

    DOI: 10.1016/j.jff.2022.105263

    Sample: serum
  4. Food & Function (2021) IF: 5.396
    Pediococcus acidilactici CCFM6432 mitigates chronic stress-induced anxiety and gut microbial abnormalities

    DOI: 10.1039/D1FO01608C

    PMID: 34704999

    Sample: serum
  5. PHYTOMEDICINE (2020) IF: 4.268
    Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics

    DOI: 10.1016/j.phymed.2020.153436

    Sample: serum
  6. Scientific Reports (2021) IF: 4.38
    The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation

    DOI: 10.1038/s41598-021-82604-7

    PMID: 33542312

    Sample: Serum,Tissue homogenate(hypothalamus,hypophysis )
    Antidepressant activity of vorinostat is associated with amelioration of oxidative stress and inflammation in a corticosterone-induced chronic stress model in mice

    DOI: 10.1016/j.bbr.2018.02.009

    PMID: 29452193

    Sample: Serum
  8. HORMONES AND BEHAVIOR (2017) IF: 3.378
    What is stressful for females? Differential effects of unpredictable environmental or social stress in CD1 female mice

    DOI: 10.1016/j.yhbeh.2017.11.013

    Sample: plasma
  9. Scientific Reports (2020) IF: 3.998
    Long term transcriptional and behavioral effects in mice developmentally exposed to a mixture of endocrine disruptors associated with delayed human neurodevelopment

    DOI: 10.1038/s41598-020-66379-x

    PMID: 32518293

    Sample: Serum
    High activity of the endogenous opioid system and acute but not chronic stress influence experimental colitis development in mice

    DOI: 10.26402/jpp.2018.5.11

    Sample: plasma
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue homogenate
  12. Sleep and Biological Rhythms (2018) IF: 0.655
    Evaluation of behavior and endocrinological changes after REM sleep deprivation-induced mania-like behavior in mice

    DOI: 10.1007/s41105-018-0166-6

    Sample: serum
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