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Mouse AST(Aspartate Aminotransferase) ELISA Kit

  • Cat.No.:E-EL-M0160

  • Reactivity: Mouse

To Purchase E-EL-M0160

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse AST in samples. No significant cross-reactivity or interference between Mouse AST and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse AST concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AST. You can calculate the concentration of Mouse AST in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse AST were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse AST were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.49 0.85 4.61 0.46 0.79 4.49
Standard deviation 0.03 0.04 0.17 0.02 0.03 0.23
CV (%) 6.12 4.71 3.69 4.35 3.80 5.12

Recovery

The recovery of Mouse AST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-108 101
EDTA plasma (n=8) 90-102 97
Cell culture media (n=8) 88-101 95

Linearity

Samples were spiked with high concentrations of Mouse AST and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P05201 
SynonymsSGOT, serum glutamic oxaloacetic transaminase, GOT1, AspAT, ASAT, AAT

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Hepatology (2017) IF: 13.246
    MiR-15a/16-1 Suppresses AHR-dependent IL-22 Secretion In CD4+ T cells And Contributes To Immune-mediated Organ Injury.

    DOI: 10.1002/hep.29573

    PMID: 29023933

    Sample: Serum
  2. Food Chemistry (2013) IF: 4.529
    Dietary Supplementation With Purified Mulberry ( Morus Australis, Poir) Anthocyanins Suppresses Body Weight Gain In High-Fat Diet Fed C57Bl/6 Mice.

    DOI: 10.1016/j.foodchem.2013.03.046

    PMID: 23768383

    Sample: Serum
  3. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    miR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model

    DOI: 10.26355/eurrev_202009_23063

    PMID: 33015817

    Sample: Serum
  4. Life Sciences (2020) IF: 3.647
    Pretreatment of exosomes derived from hUCMSCs with TNF-α ameliorates acute liver failure by inhibiting the activation of NLRP3 in macrophage

    DOI: 10.1016/j.lfs.2020.117401

    PMID: 32035931

    Sample: Whole Blood,Tissue homogenate
  5. FEBS Letters (2020) IF: 3.057
    Islet-cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice

    DOI: 10.1002/1873-3468.13859

    PMID: 32531799

    Sample: Serum
  6. Toxicology Letters (2017) IF: 3.858
    Hesperetin Derivative-14 Alleviates Inflammation By Activating PPAR-γ In Mice With CCL4-Induced Acute Liver Injury And LPS-Treated RAW264. 7 Cells.

    DOI: 10.1016/j.toxlet.2017.04.008

    PMID: 28428136

    Sample: Serum,Cell culture supernatant
  7. Preprint (2020)
    Ileal bile acid transporter inhibitor improves hepatic steatosis by changing the gut microbiota dysbiosis in NAFLD model mice

    DOI: 10.21203/rs.3.rs-21966/v1

    PMID: 99999999

    Sample: Serum
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