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Mouse β-CTx(Beta Crosslaps) ELISA Kit

  • Cat.No.:E-EL-M0372

  • Reactivity: Mouse

To Purchase E-EL-M0372

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes β-CTx in samples. No significant cross-reactivity or interference between β-CTx and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of β-CTx concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-CTx. During the reaction, β-CTx in the sample or standard competes with a fixed amount of β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level β-CTx were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level β-CTx were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 264.20 768.90 1936.80 252.10 764.90 1935.40
Standard deviation 16.10 34.60 93.00 14.60 32.90 71.60
CV (%) 6.09 4.50 4.80 5.79 4.30 3.70


The recovery of β-CTx spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 94
EDTA plasma(n=8) 91-104 98
Cell culture media(n=8) 85-96 91


Samples were spiked with high concentrations of Mouse β-CTx and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonymsb-CTx, bCTXI, bCTX-I, B-Cr, BCL, Type I Collagen C-Telopeptide-Related Fraction
Research AreaSignal transduction, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of the American Chemical Society (2020) IF: 14.612
    Smart Nanosacrificial Layer on the Bone Surface Prevents Osteoporosis through Acid–Base Neutralization Regulated Biocascade Effects

    DOI: 10.1021/jacs.0c07309

    PMID: 32960592

    Sample: Serum
    Thiaplakortone B attenuates RANKL‐induced NF‐κB and MAPK signaling and dampens OVX‐induced bone loss in mice

    DOI: 10.1016/j.biopha.2022.113622

    Sample: serum
  3. Cell Death & Disease (2020) IF: 6.304
    PKC-δ deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast–osteoblast uncoupling

    DOI: 10.1038/s41419-020-02947-3

    Sample: Serum
    Discoidin domain receptor 2 facilitates prostate cancer bone metastasis via regulating parathyroid hormone-related protein

    DOI: 10.1016/j.bbadis.2014.04.018

    Sample: Cell culture supernatant
    Cedrol, a Ginger-derived sesquiterpineol, suppresses estrogen-deficient osteoporosis by intervening NFATc1 and reactive oxygen species

    DOI: 10.1016/j.intimp.2023.109893

    Sample: serum
    Hymenialdisine: A Marine Natural Product That Acts on Both Osteoblasts and Osteoclasts and Prevents Estrogen-Dependent Bone Loss in Mice

    DOI: 10.1002/jbmr.4025

    Sample: serum
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