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Mouse β-EP(Beta-Endorphin) ELISA Kit

  • Cat.No.:E-EL-M0184

  • Reactivity: Mouse

To Purchase E-EL-M0184

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level β-EP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level β-EP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.40 99.80 404.50 51.10 94.90 411.30
Standard deviation 2.90 5.50 14.20 3.20 5.40 14.80
CV (%) 5.87 5.51 3.51 6.26 5.69 3.60


The recovery of β-EP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-105 96
EDTA plasma(n=8) 85-101 92
Cell culture media(n=8) 95-107 101


Samples were spiked with high concentrations of Mouse β-EP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01193
Research AreaCancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicines (2021) IF: 6.081
    Analgesic and Anti-Inflammatory Effects of the Synthetic Neurosteroid Analogue BNN27 during CFA-Induced Hyperalgesia

    DOI: 10.3390/biomedicines9091185

    PMID: 34572370

    Sample: serum
  2. NEUROPHARMACOLOGY (2017) IF: 5.012
    Delta-opioid receptor antagonism leads to excessive ethanol consumption in mice with enhanced activity of the endogenous opioid system

    DOI: 10.1016/j.neuropharm.2017.03.016

    Sample: Plasma
    Lymphocytes and Met-Enkephalin

    DOI: 10.1007/s12035-018-1176-8

    Sample: Plasma
  4. NEUROPHARMACOLOGY (2018) IF: 4.249
    Bidirectional selection for high and low stress-induced analgesia affects G-protein activity

    DOI: 10.1016/j.neuropharm.2018.10.014

    PMID: 30326238

    Sample: Tissue homogenate
  5. Molecular Pain (2018) IF: 3.205
    Lithium reverses mechanical allodynia through a mu opioid-dependent mechanism:

    DOI: 10.1177/1744806917754142

    Sample: brain,plasma
    G protein coupled receptor kinase-2 upregulation causes κ-opioid receptor desensitization in diabetic heart

    DOI: 10.1016/j.bbrc.2016.11.090

    Sample: Plasma
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