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Mouse BNP(Brain Natriuretic Peptide) ELISA Kit

  • Cat.No.:E-EL-M0204

  • Reactivity: Mouse

To Purchase E-EL-M0204

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.40 130.40 492.60 52.70 130.30 444.70
Standard deviation 3.20 7.20 22.70 2.70 7.80 17.30
CV (%) 6.23 5.52 4.61 5.12 5.99 3.89

Recovery

The recovery of BNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 95
EDTA plasma(n=8) 95-108 100
Cell culture media(n=8) 91-105 98

Linearity

Samples were spiked with high concentrations of Mouse BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P40753
SynonymsNPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B
Research AreaCancer, Cardiovascular, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Cardiovascular Medicine (2022) IF: 5.846
    Role of the Gut Microbiota in Glucose Metabolism During Heart Failure

    DOI: 10.3389/fcvm.2022.903316

    PMID: 35859583

    Sample: Serum
  2. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2022) IF: 2.963
    Knockout of Cardiac Troponin I-Interacting Kinase leads to cardiac dysfunction and remodeling

    DOI: 10.1111/1440-1681.13699

    Sample: Serum
  3. JOURNAL OF ENDOCRINOLOGY (2014) IF: 3.586
    PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure.

    DOI: 10.1530/joe-14-0316

    PMID: 25056115

    Sample: Plasma
  4. Biomedicines (2022) IF: 4.757
    Tetrahydrobiopterin (BH4) Supplementation Prevents the Cardiorenal Effects of Diabetes in Mice by Reducing Oxidative Stress, Inflammation and Fibrosis

    DOI: 10.3390/biomedicines10102479

    PMID: 36289741

    Sample: Plasma
  5. Pharmaceuticals (2022) IF: 5.215
    Long-Term Sleep Deprivation-Induced Myocardial Remodeling and Mitochondrial Dysfunction in Mice Were Attenuated by Lipoic Acid and N-Acetylcysteine

    DOI: 10.3390/ph16010051

    Sample: Serum
  6. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A

    DOI: 10.1111/jcmm.17160

    PMID: 35083842

    Sample: Plasma
  7. Journal of the American Heart Association (2021) IF: 5.501
    Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure

    DOI: 10.1161/JAHA.120.019961

    PMID: 33759549

    Sample: Plasma
  8. Journal of Translational Medicine (2021) IF: 5.531
    microRNA-193-3p attenuates myocardial injury of mice with sepsis via STAT3/HMGB1 axis

    DOI: 10.1186/s12967-021-03022-x

    PMID: 34503521

    Sample: Serum
  9. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

    DOI: 10.1016/j.biopha.2021.112133

    PMID: 34474337

    Sample: Serum
  10. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

    DOI: 10.1016/j.biopha.2021.111531

    PMID: 34311530

    Sample: Serum
  11. ACTA PHARMACOLOGICA SINICA (2022) IF: 7.169
    Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling

    DOI: 10.1038/s41401-022-01025-y

    Sample: Serum
  12. Acta Pharmaceutica Sinica B (2022) IF: 14.903
    Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms

    DOI: 10.1016/j.apsb.2022.11.012

    Sample: Heart
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