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Mouse COL1(Collagen Type Ⅰ) ELISA Kit

  • Cat.No.:E-EL-M0325

  • Reactivity: Mouse

To Purchase E-EL-M0325

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse COL1 in samples. No significant cross-reactivity or interference between Mouse COL1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse COL1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL1. You can calculate the concentration of Mouse COL1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse COL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse COL1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.02 2.49 9.30 1.12 2.27 10.16
Standard deviation 0.05 0.13 0.28 0.06 0.12 0.42
CV (%) 4.90 5.22 3.01 5.36 5.29 4.13


The recovery of Mouse COL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 96
EDTA plasma (n=8) 85-100 91
Cell culture media (n=8) 93-109 101


Samples were spiked with high concentrations of Mouse COL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaSignal Transduction,Stem Cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Aging-US (2020) IF: 4.831
    The role of Hrd1 in ultraviolet (UV) radiation induced photoaging

    DOI: 10.18632/aging.103851

    PMID: 33168784

    Sample: Serum
    Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing

    DOI: 10.1016/j.ijbiomac.2020.05.011

    Sample: Skin
  3. Journal of Ayurveda and Integrative Medicine (2017)
    Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers

    DOI: 10.1016/j.jaim.2017.04.005

    Sample: Calvariae Origin Osteoblastic Cell Line Subclone 4
  4. Oncology Letters (2018) IF: 1.664
    SMOC1 silencing suppresses the angiotensin II‑induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway

    DOI: 10.3892/ol.2018.8989

    Sample: Plasmid
    MicroRNA-455–3p promotes osteoblast differentiation via targeting HDAC2

    DOI: 10.1016/j.injury.2022.08.047

    Sample: Mc3T3-E1 Cell
  6. Biomed Research International (2023) IF: 3.246
    Long Noncoding RNA NORAD Promotes Fracture Healing through Interacting with Osteoblast Differentiation via Targeting miR-26a

    DOI: 10.1155/2023/9950037

    Sample: Mc3T3-E1 Cell
    Salvia miltiorrhiza Restrains Reactive Oxygen Species-Associated Pulmonary Fibrosis via Targeting Nrf2-Nox4 Redox Balance

    DOI: 10.1142/S0192415X19500575

    Sample: Lung
  8. Parasites & Vectors (2021) IF: 3.876
    In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

    DOI: 10.1186/s13071-021-04952-7

    PMID: 34488852

    Sample: Lymphocyte
  9. Tissue Engineering and Regenerative Medicine (2022) IF: 4.169
    IL-33 Promotes ST2-Dependent Fibroblast Maturation via P38 and TGF-β in a Mouse Model of Epidural Fibrosis

    DOI: 10.1007/s13770-021-00425-1

    PMID: 35195855

    Sample: Spine Scar
  10. Frontiers in Immunology (2020) IF: 5.085
    Epithelial-Mesenchymal Transition in Asthma Airway Remodeling Is Regulated by the IL-33/CD146 Axis

    DOI: 10.3389/fimmu.2020.01598

    PMID: 32793232

    Sample: Tissue Homogenate
    Metformin attenuates post‐epidural fibrosis by inhibiting the TGF‐β1/Smad3 and HMGB1/TLR4 signaling pathways

    DOI: 10.1111/jcmm.16398

    Sample: Cell(Mcf-7 Cancer Cells)
  12. Frontiers in Pharmacology (2018) IF: 5.811
    A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2

    DOI: 10.3389/fphar.2018.00628

    Sample: Cell Culture Supernatant
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