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Mouse CXCL15(Chemokine C-X-C-Motif Ligand 15) ELISA Kit

Citations(4)Uniprot : Q9WVL7

Cat.No.:E-EL-M0269
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	3.5h
Detection range	78.13-5000 pg/mL
Sensitivity	46.88 pg/mL
Sample type &Sample volume	Serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse CXCL15 in samples. No significant cross-reactivity or interference between Mouse CXCL15 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse CXCL15 concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CXCL15. You can calculate the concentration of Mouse CXCL15 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CXCL15 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CXCL15 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	236.71	548.76	2092.72	259.34	580.06	1969.19
Standard deviation	14.20	25.57	91.66	15.33	29.29	72.07
CV (%)	6.00	4.66	4.38	5.91	5.05	3.66

Recovery

The recovery of Mouse CXCL15 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-107	98
EDTA plasma (n=8)	93-109	100
Cell culture media (n=8)	87-99	92

Target Information

Database Links	SwissProt: Q9WVL7
Synonyms	C-X-C motif chemokine 15,Chemokine (C-X-C motif) ligand 15,Cxcl15,CXL15,Il8,Lungkine,Scyb15,Small inducible cytokine B15,Small inducible cytokine subfamily B member 15,Small-inducible cytokine B15,weche
Research Area	Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Bioengineered (2021) IF: 3.269

The protective effects of Pimavanserin against cerebral ischemia-induced brain injury

DOI: 10.1080/21655979.2021.1978617
PMID: 34605354	Sample: Brain Tissue

American Journal of Translational Research (2020) IF: 3.375

Overexpression of lncRNA Gm43050 alleviates apoptosis and inflammation response induced by sevoflurane treatment by regulating miR-640/ZFP91


PMID: 32913509	Sample: Primary Hippocampal Neurons

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2021) IF: 4.101

FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice

DOI: 10.3892/ijmm.2021.4920
PMID: 33760144	Sample: Femoral Vein Tissue,Plasma

Stem Cell Research & Therapy (2021) IF: 6.832

Human placenta mesenchymal stem cell-derived exosomes delay H2O2-induced aging in mouse cholangioids

DOI: 10.1186/s13287-021-02271-3
	Sample: Organoids

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