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Mouse Cys-C(Cystatin C) ELISA Kit

Citations(14)Uniprot : P21460

Cat.No.:E-EL-M3024
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse Cys-C in samples. No significant cross-reactivity or interference between Mouse Cys-C and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse Cys-C concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Cys-C. You can calculate the concentration of Mouse Cys-C in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Cys-C were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Cys-C were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	48.88	105.09	456.05	40.09	123.09	409.82
Standard deviation	2.55	4.76	20.89	2.29	5.97	17.95
CV (%)	5.22	4.53	4.58	5.71	4.85	4.38

Recovery

The recovery of Mouse Cys-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-102	99
EDTA plasma (n=8)	89-101	94
Cell culture media (n=8)	84-96	91

Linearity

Samples were spiked with high concentrations of Mouse Cys-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P21460
Synonyms	CST3, Cystatin 3, ARMD11, Post-Gamma-Globulin
Research Area	Cancer, Cell Biology, Cardiovascular, Stem cells, Tags and Cell Markers

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Redox Biology (2019) IF: 11.799
Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway

DOI: 10.1016/j.redox.2019.101411

PMID: 31884071
Sample: Serum
Journal of Translational Medicine (2022) IF: 8.44
SGLT2 inhibitors improve kidney function and morphology by regulating renal metabolic reprogramming in mice with diabetic kidney disease

DOI: 10.1186/s12967-022-03629-8

PMID: 36104729
Sample: serum
Journal of Translational Medicine (2022) IF: 8.44
Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism

DOI: 10.1186/s12967-022-03717-9

Sample: serum
Translational Research (2022) IF: 7.012
Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential mortality prediction in human

DOI: 10.1016/j.trsl.2022.01.002

PMID: 35077866
Sample: plasma
Journal of Inflammation Research (2021) IF: 6.922
Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

DOI: 10.2147/JIR.S301104

Sample: Serum,Plasma,Urine
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Calcium dobesilate mediates renal interstitial fibrosis and delay renal peritubular capillary loss through Sirt1/p53 signaling pathway

DOI: 10.1016/j.biopha.2020.110798

PMID: 33011612
Sample: Serum
INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
Ginkgolide A attenuates sepsis-associated kidney damage via upregulating microRNA-25 with NADPH oxidase 4 as the target

DOI: 10.1016/j.intimp.2021.107514

PMID: 33677255
Sample: Serum
KIDNEY & BLOOD PRESSURE RESEARCH (2018) IF: 3
Rotenone Protects Against Acetaminophen-Induced Kidney Injury by Attenuating Oxidative Stress and Inflammation

DOI: 10.1159/000492589

Sample: serum
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2019) IF: 3.323
ADAMTS13 protects mice against renal ischemia-reperfusion injury by reducing inflammation and improving endothelial function

DOI: 10.1152/ajprenal.00405.2018

PMID: 30461292
Sample: Plasma,Tissue homogenate
TOXICOLOGY LETTERS (2020) IF: 3.569
Local renal complement activation mediates immune kidney injury by inducing endothelin-1 signalling and inflammation in trichloroethylene-sensitised mice

DOI: 10.1016/j.toxlet.2020.07.036

Sample: serum
PEPTIDES (2021) IF: 3.75
Alamandine, a derivative of angiotensin-(1-7), alleviates sepsis-associated renal inflammation and apoptosis by inhibiting the PI3K/Ak and MAPK pathways

DOI: 10.1016/j.peptides.2021.170627

PMID: 34400214
Sample: serum
MOLECULAR BIOLOGY REPORTS (2022) IF: 2.316
Therapeutic effect of lycopene in lipopolysaccharide nephrotoxicity through alleviation of mitochondrial dysfunction, inflammation, and oxidative stress

DOI: 10.1007/s11033-022-07661-1

PMID: 35672542
Sample: serum
Nanoscale Research Letters (2016) IF: 2.584
The Potential Application of Raw Cadmium Sulfide Nanoparticles as CT Photographic Developer

DOI: 10.1186/s11671-016-1424-7

Sample: Serum
JOURNAL OF TOXICOLOGICAL SCIENCES (2020) IF: 1.737
Intracellular complement activation in podocytes aggravates immune kidney injury in trichloroethylene-sensitized mice

DOI: 10.2131/jts.45.681

PMID: 33132242
Sample: Serum

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