Mouse ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit (E-EL-M0465)

Name: Mouse ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit
Brand: Elabscience
SKU: E-EL-M0465
Price: 150.0 USD
Availability: InStock

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Mouse ECF/CCL11(Eosinophil Chemotactic Factor) ELISA Kit - 1

Size	Price	Qty
96T	$ 700.00	- +
48T	$ 595.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse Eotaxin 1/CCL11 in samples.No significant cross-reactivity or interference between Mouse Eotaxin 1/CCL11 and analogues was observed
Recovery	80%-120%
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.670	2.670	2.670	2.594
500	1.632	1.678	1.655	1.579
250	0.965	0.929	0.947	0.871
125	0.507	0.521	0.514	0.438
62.5	0.303	0.283	0.293	0.217
31.25	0.204	0.188	0.196	0.120
15.63	0.136	0.144	0.140	0.064
0	0.068	0.084	0.076	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.100	114.270	351.620	45.420	109.930	375.720
Standard deviation	2.960	5.610	15.010	2.720	5.490	12.060
CV（%）	6.280	4.910	4.270	5.980	4.990	3.210

Recovery

The recovery of Mouse ECF/CCL11 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	91-106	98
Serum （n=8）	94-107	99
EDTA plasma （n=8）	85-96	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse ECF/CCL11 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-112	85-99	84-97
1:2	Average	103	92	91
1:4	Range	92-104	86-100	86-98
1:4	Average	99	92	92
1:8	Range	94-106	82-97	81-92
1:8	Average	99	89	86

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	95.62-101.42
Sample 2（n=16）	87.37-93.24

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Eotaxin 1/CCL11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Eotaxin 1/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Eotaxin 1/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Eotaxin 1/CCL11. You can calculate the concentration of Mouse Eotaxin 1/CCL11 in the samples by comparing the OD of the samples to the standard curve.

Background

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce angiogenic responses by CCR3-positive microvascular endothelial cells.