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Mouse FGF21(Fibroblast Growth Factor 21) ELISA Kit

Citations(6)Uniprot : Q9JJN1

Cat.No.:E-EL-M0029
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse FGF21 in samples. No significant cross-reactivity or interference between Mouse FGF21 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse FGF21 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGF21, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FGF21. You can calculate the concentration of Mouse FGF21 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse FGF21 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse FGF21 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	107.57	248.69	885.55	106.49	258.75	964.05
Standard deviation	6.07	10.57	46.49	6.40	10.45	40.10
CV (%)	5.64	4.25	5.25	6.01	4.04	4.16

Recovery

The recovery of Mouse FGF21 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-106	99
EDTA plasma (n=8)	85-101	92
Cell culture media (n=8)	86-98	92

Linearity

Samples were spiked with high concentrations of Mouse FGF21 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9JJN1
Synonyms	FGF-21
Research Area	Cancer, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Hepatology Communications (2023) IF: 5.701
Canagliflozin ameliorates the development of NAFLD by preventing NLRP3-mediated pyroptosis through FGF21-ERK1/2 pathway

DOI: 10.1097/HC9.0000000000000045

Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
Pyruvate Upregulates Hepatic FGF21 Expression by Activating PDE and Inhibiting cAMP–Epac–CREB Signaling Pathway

DOI: 10.3390/ijms23105490

Sample: AML12 hepatocyte
Journal of Diabetes (2019) IF: 3.298
Renoprotective effects of brown adipose tissue activation in diabetic mice

DOI: 10.1111/1753-0407.12938

PMID: 31020790
Sample: Serum,Urine
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
MicroRNA‑100‑5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21

DOI: 10.3892/ijmm.2018.4017

Sample: Serum
Open Life Sciences (2022) IF: 1.311
β-Hydroxybutyrate upregulates FGF21 expression through inhibition of histone deacetylases in hepatocytes

DOI: 10.1515/biol-2022-0095

Sample: serum
Food Science & Nutrition (2020) IF: 1.797
The protective effect of sulforaphane on type II diabetes induced by high‐fat diet and low‐dosage streptozotocin

DOI: 10.1002/fsn3.2040

Sample: serum

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