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Mouse FSH(Follicle Stimulating Hormone) ELISA Kit

  • Cat.No.:E-EL-M0511

  • Reactivity: Mouse

To Purchase E-EL-M0511

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse FSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse FSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse FSH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.70 8.90 43.10 5.10 8.40 40.20
Standard deviation 0.30 0.40 2.10 0.30 0.40 1.70
CV (%) 6.38 4.49 4.87 5.88 4.76 4.23

Recovery

The recovery of Mouse FSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma (n=8) 89-106 97
Cell culture media (n=8) 89-104 96

Linearity

Samples were spiked with high concentrations of Mouse FSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsFSH

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell death & disease (2019) IF: 5.638
    Germline deletion of Cdyl causes teratozoospermia and progressive infertility in male mice

    DOI: 10.1038/s41419-019-1455-y

    PMID: 30850578

    Sample: Serum
  2. Scientific Reports (2017) IF: 4.259
    Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy.

    DOI: 10.1038/srep43475

    PMID: 28276438

    Sample: Serum
  3. Journal of Biochemical and Molecular Toxicology (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide-induced premature ovarian failure

    DOI: 10.1002/jbt.22591

    PMID: 32711407

    Sample: Serum
  4. Toxicology Letters (2014) IF: 3.858
    Differential Proteome And Gene Expression Reveal Response To Carbon Ion Irradiation In Pubertal Mice Testes.

    DOI: 10.1016/j.toxlet.2014.01.001

    PMID: 24440814

    Sample: Serum
  5. Molecular Reproduction and Development (2020) IF: 2.823
    Adoptive transfers of CD4 + CD25 + Tregs partially alleviate mouse premature ovarian insufficiency

    DOI: 10.1002/mrd.23404

    PMID: 32741069

    Sample: Serum
  6. Drug and Chemical Toxicology (2019) IF: 2.405
    Fluted pumpkin seeds protect against busulfan-induced oxidative stress and testicular injuries in adult mice

    DOI: 10.1080/01480545.2019.1657885

    PMID: 31469002

    Sample: Plasma
  7. Reproductive Biology and Endocrinology (2018) IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

    DOI: 10.1186/s12958-018-0404-4

    PMID: 30176887

    Sample: Plasma
  8. IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage
    Sample: Serum
  9. Reproductive Biology and Endocrinology (2018) IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

    DOI: 10.1186/s12958-018-0404-4

    PMID: 30176887

    Sample: Serum
  10. Reproductive Biology and Endocrinology (2018) IF: 2.852
    The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.

    DOI: 10.1186/s12958-018-0365-7

    PMID: 29728128

    Sample: Serum
  11. Reproductive Biology and Endocrinology (2018) IF: 2.852
    The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model.

    DOI: 10.1186/s12958-018-0362-x

    PMID: 29751768

    Sample: Serum
  12. Plos One (2015) IF: 2.806
    Diet-Induced Obesity In Male C57BL/6 Mice Decreases Fertility As A Consequence Of Disrupted Blood-Testis Barrier.

    DOI: 10.1371/journal.pone.0120775

    PMID: 25886196

    Sample: Serum
  13. Tropical Journal of Pharmaceutical Research (2019) IF: 0.504
    Effect of total flavonoids of Cuscuta chinensis Lam. (Convolvulaceae) on oxidative stress injury in mouse testis and epididymis, and on serum levels of reproductive hormones in oligoasthenospermia mice model

    DOI: 10.4314/tjpr.v18i6.15

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  14. Preprint (2020)
    Elevated levels of miR-483-5p in oocytes aggravates premature ovarian insufficiency induced by CDDP by targeting the FKBP4

    DOI: 10.21203/rs.3.rs-17131/v1

    PMID: 99999999

    Sample: Serum
  15. Heliyon (2020)
    The intake of an extract from seeds of Tamarindus indica L. modulates the endocrine function of adult male mice under a high fat diet

    DOI: 10.1016/j.heliyon.2020.e03310

    PMID: 32051875

    Sample: Serum
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