Mouse GAL3(Galectin 3) ELISA Kit (E-EL-M0529)

Name: Mouse GAL3(Galectin 3) ELISA Kit
Brand: Elabscience
SKU: E-EL-M0529
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GAL3 in samples. No significant cross-reactivity or interference between Mouse GAL3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.380	2.416	2.398	2.319
1000	1.559	1.581	1.570	1.491
500	0.990	0.954	0.972	0.893
250	0.470	0.494	0.482	0.403
125	0.289	0.275	0.282	0.203
62.5	0.186	0.174	0.180	0.101
31.25	0.128	0.134	0.131	0.052
0	0.070	0.088	0.079	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	96.260	206.970	759.710	88.980	200.630	725.750
Standard deviation	4.840	11.240	26.440	5.300	10.650	25.470
CV（%）	5.030	5.430	3.480	5.960	5.310	3.510

Recovery

The recovery of Mouse GAL3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	93-110	101
Serum （n=8）	93-108	99
EDTA plasma （n=8）	87-99	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GAL3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-101	86-98	91-104
1:2	Average	92	91	96
1:4	Range	85-99	85-95	90-103
1:4	Average	92	90	97
1:8	Range	85-99	84-96	88-102
1:8	Average	90	90	93

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	90.97-108.88
Sample 2（n=16）	85.12-89.08

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GAL3. You can calculate the concentration of Mouse GAL3 in the samples by comparing the OD of the samples to the standard curve.

Background

Galectin-3 is a member of the lectin family, which includes 15 mammalian lectin members. Like all galectins, galectin-3 contains a carbohydrate recognition binding domain of about 130 amino acids that enables the specific binding of β-galactosides. This protein has been shown to be involved in cell adhesion, cell activation, chemoattraction, cell growth and differentiation, cell cycle, and apoptosis. Galectin-3 induces pro-inflammatory cytokines and inhibits Th2 type cytokine production. It chemoattracts monocytes and macrophages, and activates and degranulates basophils and mast cells. Galectin-3 has been shown to be involved in cancer, inflammation, fibrosis, heart disease, and stroke. It has also been shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, tissue repair, inflammation, fibrogenesis, and ventricular remodeling. In addition, galectin-3 was found to contribute to tumor cell evasion of the immune system. Galectin-3 is increasingly being used as a diagnostic marker for different cancers.