Mouse GHRL(Ghrelin) ELISA Kit (E-EL-M0551)

Name: Mouse GHRL(Ghrelin) ELISA Kit
Brand: Elabscience
SKU: E-EL-M0551
Price: 150.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.1 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GHRL in samples.No significant cross-reactivity or interference between Mouse GHRL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	0.152	0.152	0.152	-
5	0.219	0.221	0.220	-
2.5	0.370	0.348	0.359	-
1.25	0.603	0.591	0.597	-
0.63	0.910	0.888	0.899	-
0.31	1.214	1.236	1.225	-
0.16	1.582	1.512	1.547	-
0	2.099	2.149	2.124	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.470	1.010	3.360	0.460	0.960	3.640
Standard deviation	0.030	0.050	0.140	0.030	0.080	0.260
CV（%）	6.200	4.840	4.130	6.760	8.580	7.200

Recovery

The recovery of Mouse GHRL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-105	97
EDTA plasma （n=8）	90-107	97
Cell culture media （n=8）	87-99	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GHRL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-111	96-108	95-111
1:2	Average	102	103	103
1:4	Range	96-108	91-103	89-101
1:4	Average	102	97	95
1:8	Range	99-117	87-100	90-102
1:8	Average	107	94	96

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	85.92-113.87
Sample 2（n=16）	86.81-89.31

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GHRL. During the reaction, Mouse GHRL in the sample or standard competes with a fixed amount of Mouse GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

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