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Mouse HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-M0687

  • Reactivity: Mouse

To Purchase E-EL-M0687

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 125.00-8000 pg/mL
Sensitivity 75.00 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse HIF-1α in samples. No significant cross-reactivity or interference between Mouse HIF-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse HIF-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HIF-1α. You can calculate the concentration of Mouse HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HIF-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HIF-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 387.70 1086.20 2742.50 371.80 1120.30 2531.80
Standard deviation 24.80 46.70 109.70 19.70 58.30 108.90
CV (%) 6.40 4.30 4.00 5.30 5.20 4.30


The recovery of Mouse HIF-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma (n=8) 93-105 99
Cell culture media (n=8) 89-102 95


Samples were spiked with high concentrations of Mouse HIF-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q61221
SynonymsHIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ADVANCED MATERIALS (2022) IF: 32.086
    Supramolecular Nitric Oxide Depot for Hypoxic Tumor Vessel Normalization and Radiosensitization

    DOI: 10.1002/adma.202202625

    PMID: 35906003

    Sample: B16 cell-xenograft
    Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

    DOI: 10.1016/j.jid.2021.04.019

    PMID: 34029575

    Sample: Serum,Tissue homogenate
    Sinomenine mitigates collagen-induced arthritis mice by inhibiting angiogenesis

    DOI: 10.1016/j.biopha.2019.108759

    PMID: 30856539

    Sample: Serum
  4. Journal of Inflammation Research (2021) IF: 6.922
    Histone Deacetylase 6 Regulates the Activation of M1 Macrophages by the Glycolytic Pathway During Acute Liver Failure

    DOI: 10.2147/JIR.S302391

    Sample: liver,RAW264.7 cell
    Amelioration of diabetic retinopathy in db/db mice by treatment with different proportional three active ingredients from Tibetan medicine Berberis dictyophylla F

    DOI: 10.1016/j.jep.2021.114190

    PMID: 33964362

    Sample: Serum
    Uncovering the Metabolic Mechanism of Salidroside Alleviating Microglial Hypoxia Inflammation Based on Microfluidic Chip-Mass Spectrometry

    DOI: 10.1021/acs.jproteome.1c00647

    PMID: 34851127

    Sample: BV2 cell
  7. Stem Cell Research & Therapy (2018) IF: 4.963
    LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning

    DOI: 10.1186/s13287-018-1031-x

    PMID: 30359325

    Sample: Cell culture supernatant
    MLC901 Favors Angiogenesis and Associated Recovery after Ischemic Stroke in Mice

    DOI: 10.1159/000444810

    Sample: Tissue homogenate
  9. INFLAMMATION (2018) IF: 2.884
    RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice

    DOI: 10.1007/s10753-018-0815-6

    PMID: 30043119

    Sample: Serum
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

    DOI: 10.3967/bes2018.101

    Sample: Plasma,Tissue homogenate
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