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Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit

  • Cat.No.:E-EL-M0676

  • Reactivity: Mouse

To Purchase E-EL-M0676

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse HMGB-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.50 98.56 334.18 52.21 95.09 337.87
Standard deviation 2.65 4.51 17.78 3.53 5.30 13.18
CV (%) 5.25 4.58 5.32 6.76 5.57 3.90

Recovery

The recovery of Mouse HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 99
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 83-98 90

Linearity

Samples were spiked with high concentrations of Mouse HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P63158
SynonymsHMGB1, HMG1, HMG3, SBP-1, Amphoterin

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Adv. Funct. Mater. (2020) IF: 16.836
    Inducing Endoplasmic Reticulum Stress to Expose Immunogens: A DNA Tetrahedron Nanoregulator for Enhanced Immunotherapy

    DOI: DOI: 10.1002/adfm.202000532

    PMID: 99999999

    Sample: Serum
  2. IF: 15.624
    Transformable Nanoparticle-Enabled Synergistic Elicitation and Promotion of Immunogenic Cell Death for Triple-Negative Breast Cancer Immunotherapy
    Sample: Cell supernatant
  3. Advanced Functional Materials (2019) IF: 15.624
    Transformable Nanoparticle-Enabled Synergistic Elicitation and Promotion of Immunogenic Cell Death for Triple-Negative Breast Cancer Immunotherapy

    DOI: 10.1002/adfm.201905213

    Sample: Cell culture supernatant
  4. Journal of Nanobiotechnology (2019) IF: 5.345
    Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer

    DOI: 10.1186/s12951-019-0541-8

    PMID: 31623629

    Sample: Cell culture supernatant
  5. Laboratory Investigation (2019) IF: 4.197
    JAK2/STAT1-mediated HMGB1 translocation increases inflammation and cell death in a ventilator-induced lung injury model

    DOI: 10.1038/s41374-019-0308-8

    PMID: 31467427

    Sample: Cell lysate,Bronchoalveolar Lavage Fluid,Tissue ho
  6. Frontiers in microbiology (2019) IF: 4.259
    Galectin-3 deficiency facilitates TNF-α-dependent hepatocyte death and liver inflammation in MCMV infection

    DOI: 10.3389/fmicb.2019.00185

    PMID: 30800112

    Sample: Tissue homogenate
  7. International Immunopharmacology (2020) IF: 3.943
    miR-129-5p alleviates LPS-induced acute kidney injury via targeting HMGB1/TLRs/NF-kappaB pathway

    DOI: 10.1016/j.intimp.2020.107016

    PMID: 33039954

    Sample: Serum
  8. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    miR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model

    DOI: 10.26355/eurrev_202009_23063

    PMID: 33015817

    Sample: Serum
  9. Investigative Ophthalmology & Visual Science (2020) IF: 3.47
    Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis

    DOI: 10.1167/iovs.61.4.48

    PMID: 32347916

    Sample: Cell culture supernatant
  10. American Journal of Physiology-Renal Physiology (2018) IF: 3.164
    SIRT1-mediated HMGB1 deacetylation suppresses sepsis-associated acute kidney injury

    DOI: 10.1152/ajprenal.00119.2018

    PMID: 30379096

    Sample: Serum
  11. International Journal of Molecular Sciences (2017) IF: 3.226
    High-Mobility Group Box 1 Disrupts Metabolic Function With Cigarette Smoke Exposure In A Ceramide-Dependent Manner.

    DOI: 10.3390/ijms18051099

    PMID: 28531105

    Sample: Serum,Cell culture supernatant
  12. Inflammation (2018) IF: 2.884
    Hydrogen-rich saline ameliorates experimental autoimmune encephalomyelitis in C57BL/6 mice via the Nrf2-ARE signaling pathway

    DOI: 10.1007/s10753-018-0915-3

    PMID: 30343391

    Sample: Tissue homogenate
  13. International Immunopharmacology (2015) IF: 2.956
    Hesperidin Ameliorates Lipopolysaccharide-Induced Acute Lung Injury In Mice By Inhibiting Hmgb1 Release.

    DOI: 10.1016/j.intimp.2015.02.022

    PMID: 25724384

    Sample: Bronchoalveolar Lavage Fluid
  14. International Immunopharmacology (2014) IF: 2.956
    Protective Effects Of Hesperidin On Concanavalin A-Induced Hepatic Injury In Mice.

    DOI: 10.1016/j.intimp.2014.05.018

    PMID: 24867793

    Sample: Serum
  15. Medical Science Monitor (2016) IF: 1.585
    Protective Role Of Grape Seed Proanthocyanidins Against Ccl4 Induced Acute Liver Injury In Mice.

    DOI: 10.12659/MSM.895552

    PMID: 26986029

    Sample: Serum
  16. Journal of Cellular and Molecular Medicine (2020)
    Cigarette smoke-induced HMGB1 translocation and release contribute to migration and NF-κB activation through inducing autophagy in lung macrophages

    DOI: 10.1111/jcmm.14789

    PMID: 31769590

    Sample: Serum,Cell culture supernatant
  17. Available at SSRN (2018)
    Hypothermic Oxygenated Machine Perfusion Alleviates Donation after Cardiac Death Liver Injury Through Regulating P-Selectin-Dependent and-Independent Pathways in Mice

    DOI: 10.1097/TP.0000000000002621

    PMID: 31033856

    Sample: Tissue homogenate
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