Current Position:Home>>Kits>>ELISA Kits>>special>> Mouse hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

Mouse hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

Citations(5)

Cat.No.:E-EL-M0677
Reactivity: Mouse

Filter:

To Purchase E-EL-M0677

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Properties

Format	24T, 48T, 96T, 96T5, 96T10
Assay time	3.5h
Sample type	serum, plasma and other biological fluids
Sample volume	100μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 6.86-5000 pg/mL Sensitivity: 4.12 pg/mL
Application	Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Reproducibility	Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse hs-CRP. You can calculate the concentration of Mouse hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

FASEB JOURNAL (2021) IF: 5.192

Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

DOI: 10.1096/fj.202101030R
PMID: 34516679	Sample: Plasma

American Journal of Translational Research (2018) IF: 4.06

Apolipoprotein B-100 peptide 210 antibody inhibits atherosclerosis by regulation of macrophages that phagocytize oxidized lipid


PMID: 30018722	Sample: Plasma

STEM CELLS AND DEVELOPMENT (2022) IF: 4.39

Alteration of transcriptomic profile and anti-septic efficacy of adipose-derived mesenchymal stromal/stem cells under different culture conditions

DOI: 10.1089/scd.2022.0238
	Sample: Plasma

Frontiers in Aging Neuroscience (2022) IF: 5.702

Transplantation of fecal microbiota from APP/PS1 mice and Alzheimer’s disease patients enhanced endoplasmic reticulum stress in the cerebral cortex of wild-type mice

DOI: 10.3389/fnagi.2022.858130
	Sample: Serum

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714

Menthone inhibits type-I interferon signaling by promoting Tyk2 ubiquitination to relieve local inflammation of rheumatoid arthritis

DOI: 10.1016/j.intimp.2022.109228
PMID: 36095947	Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha