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Mouse hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

  • Cat.No.:E-EL-M0677

  • Reactivity: Mouse

To Purchase E-EL-M0677

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609


Format 24T, 48T, 96T, 96T*5, 96T*10
Assay time 3.5h
Sample type serum, plasma and other biological fluids
Sample volume 100μL
Sample size Run up to 46 samples in duplicate and 30 samples in triplicate
Storage 2-8℃
Interpretation Detection range: 6.86-5000 pg/mL
Sensitivity: 4.12 pg/mL
Application Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Reproducibility Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse hs-CRP. You can calculate the concentration of Mouse hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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