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Mouse IFN-β(Interferon Beta) ELISA Kit

Citations(24)Uniprot : P01575

  • Cat.No.:E-EL-M0033

  • Reactivity: Mouse
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To Purchase E-EL-M0033

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IFN-β in samples. No significant cross-reactivity or interference between Mouse IFN-β and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-β concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-β. You can calculate the concentration of Mouse IFN-β in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFN-β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFN-β were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.80 98.00 486.50 50.40 89.40 514.50
Standard deviation 2.50 4.60 18.50 2.50 5.30 26.20
CV (%) 5.23 4.69 3.80 4.96 5.93 5.09

Recovery

The recovery of Mouse IFN-β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-108 99
EDTA plasma (n=8) 91-102 96
Cell culture media (n=8) 91-105 98

Linearity

Samples were spiked with high concentrations of Mouse IFN-β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01575
SynonymsIFNB1, IFB, IFF, IFNB
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. JOURNAL OF MEDICAL VIROLOGY (2022) IF: 20.693
    FBXO6 regulates the anti-viral immune-responses via mediating alveolar macrophages survival

    DOI: 10.1002/jmv.28203

    PMID: 36217277

    		 Sample: BALF
  2. ACS Nano (2023) IF: 18.027
    Sensitizing Tumors to Immune Checkpoint Blockage via STING Agonists Delivered by Tumor-Penetrating Neutrophil Cytopharmaceuticals

    DOI: 10.1021/acsnano.2c11764

    PMID: 36595464

    		 Sample: 4T1 cell-Xenograft,4T1 cell-Xenograft cell,macroph
  3. Nature Microbiology (2019) IF: 14.633
    ADP-ribosyltransferase PARP11 modulates the interferon antiviral response by mono-ADP-ribosylating the ubiquitin E3 ligase β-TrCP

    DOI: 10.1038/s41564-019-0428-3
    Sample: serum
  4. Theranostics (2022) IF: 11.6
    Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy

    DOI: 10.7150/thno.73104

    PMID: 35910792

    		 Sample: RAW 264.7 cell,BV2 cell
  5. Redox Biology (2022) IF: 11.799
    STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice

    DOI: 10.1016/j.redox.2022.102367

    PMID: 35724543

    		 Sample: serum,Kupffer cell (KCs)
  6. Nano Research (2022) IF: 10.269
    Peptide nanotube loaded with a STING agonist, c-di-GMP, enhance cancer immunotherapy against melanoma

    DOI: 10.1007/s12274-022-5102-z
  7. Cell Reports (2023) IF: 9.9949
    RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.

    DOI: 10.1016/j.celrep.2023.112097
    Sample: RAW264.7 cell
  8. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
    Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages

    DOI: 10.1016/j.ijbiomac.2022.07.043

    PMID: 35835298

    		 Sample: Raw 264.7 macrophages
  9. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
    Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation

    DOI: 10.1016/j.ijbiomac.2022.11.225
    Sample: serum
  10. Acta Biomaterialia (2021) IF: 8.9469
    Sialic acid conjugate-modified liposomes enable tumor homing of epirubicin via neutrophil/monocyte infiltration for tumor therapy

    DOI: 10.1016/j.actbio.2021.07.063

    PMID: 34339869

    		 Sample: S180 cell-Xenograft
  11. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.088
    Discovery of fusidic acid derivatives as novel STING inhibitors for treatment of sepsis

    DOI: 10.1016/j.ejmech.2022.114814

    PMID: 36228412

    		 Sample: RAW264.7 cells
  12. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.088
    Design and synthesis of hederagenin derivatives modulating STING/NF-κB signaling for the relief of acute liver injury in septic mice

    DOI: 10.1016/j.ejmech.2022.114911
  13. IMMUNOLOGY (2021) IF: 7.397
    Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3

    DOI: 10.1111/imm.13315

    PMID: 33513265

    		 Sample: Cell（HEK293T cells,the mouseperitoneal macrophage
  14. Frontiers in Immunology (2021) IF: 7.561
    Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection

    DOI: 10.3389/fimmu.2021.626110

    PMID: 33763070

    		 Sample: Cell culture supernatant
  15. PLoS Pathogens (2018) IF: 6.158
    Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome

    DOI: 10.1371/journal.ppat.1007067
    Sample: Serum
  16. International Journal of Nanomedicine (2021) IF: 6.4
    Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation

    DOI: 10.2147/IJN.S318363
    Sample: serum,CD8+ T cell,BMDCs
  17. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    Activation of the STING-IRF3 pathway involved in psoriasis with diabetes mellitus

    DOI: 10.1111/jcmm.17236
    Sample: serum
  18. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Menthone inhibits type-I interferon signaling by promoting Tyk2 ubiquitination to relieve local inflammation of rheumatoid arthritis

    DOI: 10.1016/j.intimp.2022.109228

    PMID: 36095947

    		 Sample: serum
  19. Frontiers in Microbiology (2017) IF: 4.076
    miR-194 Inhibits Innate Antiviral Immunity by Targeting FGF2 in Influenza H1N1 Virus Infection

    DOI: 10.3389/fmicb.2017.02187
    Sample: Cell culture supernatant,Bronchoalveolar Lavage Fl
  20. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2019) IF: 4.213
    Terminating the renewal of tumor-associated macrophages: A sialic acid-based targeted delivery strategy for cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2019.118706
    Sample: Tissue homogenate
  21. JOURNAL OF VIROLOGY (2020) IF: 4.501
    DDIT3 targets innate immunity via the DDIT3-OTUD1-MAVS pathway to promote BVDV replication

    DOI: 10.1128/JVI.02351-20
    Sample: serum
  22. JOURNAL OF IMMUNOLOGY (2020) IF: 4.886
    OTUD1 Negatively Regulates Type I IFN Induction by Disrupting Noncanonical Ubiquitination of IRF3

    DOI: 10.4049/jimmunol.1900305
    Sample: serum
  23. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    The selective STING inhibitor H-151 preserves myocardial function and ameliorates cardiac fibrosis in murine myocardial infarction

    DOI: 10.1016/j.intimp.2022.108658

    PMID: 35278833

    		 Sample: serum
  24. BMC IMMUNOLOGY (2022) IF: 3.594
    NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

    DOI: 10.1186/s12865-022-00515-2

    PMID: 35965334

    		 Sample: Raw 264.7 cell
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