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Mouse IgA(Immunoglobulin A) ELISA Kit

  • Cat.No.:E-EL-M0690

  • Reactivity: Mouse

To Purchase E-EL-M0690

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 6.25-400 ng/mL
Sensitivity 3.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IgA in samples. No significant cross-reactivity or interference between Mouse IgA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgA. You can calculate the concentration of Mouse IgA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 20.95 52.87 156.46 20.01 53.52 164.75
Standard deviation 1.44 2.29 8.53 1.08 3.18 7.51
CV (%) 6.87 4.33 5.45 5.40 5.94 4.56


The recovery of Mouse IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 93-110 101


Samples were spiked with high concentrations of Mouse IgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. International Journal of Nanomedicine (2022) IF: 6.4
    The Immune-Enhancing Properties of Hwanglyeonhaedok-Tang-Mediated Biosynthesized Gold Nanoparticles in Macrophages and Splenocytes

    DOI: 10.2147/IJN.S338334

    PMID: 35125869

    Sample: RAW264.7 cell,splenocytes
  2. Scientific Reports (2016) IF: 5.228
    Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice

    DOI: 10.1038/srep34079

    Sample: Serum
    Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice

    DOI: 10.1002/jsfa.12173

    PMID: 36054505

    Sample: serum,colon tissue
  4. Food & Function (2020) IF: 4.171
    Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses

    DOI: 10.1039/D0FO01841D

    PMID: 32955540

    Sample: Plasma
  5. Foods (2022) IF: 4.35
    Lactobacillus fermentum Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner

    DOI: 10.3390/foods11091229

    PMID: 35563952

    Sample: Duodenum,jejunum,ileum,colon,colon contents
  6. Journal of Functional Foods (2022) IF: 4.451
    Immune-enhancing efficacy of Curtobacterium proimmune K3 lysates isolated from Panax ginseng beverages in cyclophosphamide-induced immunosuppressed mice

    DOI: 10.1016/j.jff.2022.105020

    Sample: serum
  7. Journal of Functional Foods (2020) IF: 3.701
    Dietary supplementation of Bacillus sp. DU106 activates innate immunity and regulates intestinal microbiota in mice

    DOI: 10.1016/j.jff.2020.104247

    Sample: Serum
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557

    Sample: serum
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