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Mouse IgG(Immunoglobulin G) ELISA Kit

  • Cat.No.:E-EL-M0692

  • Reactivity: Mouse

To Purchase E-EL-M0692

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IgG in samples. No significant cross-reactivity or interference between Mouse IgG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IgG concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG. You can calculate the concentration of Mouse IgG in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgG were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.50 11.30 34.70 5.40 11.70 34.30
Standard deviation 0.40 0.50 1.80 0.30 0.60 1.20
CV (%) 7.27 4.42 5.19 5.56 5.13 3.50

Recovery

The recovery of Mouse IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-110 100
EDTA plasma (n=8) 92-107 99
Cell culture media (n=8) 90-106 97

Linearity

Samples were spiked with high concentrations of Mouse IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01863
SynonymsIgG

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. NANO LETTERS (2019) IF: 11.238
    NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment

    DOI: 10.1021/acs.nanolett.9b02501

    PMID: 31593471

    Sample: Serum
  2. Food & Function (2020) IF: 4.171
    Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses

    DOI: 10.1039/d0fo01841d

    PMID: 32955540

    Sample: Plasma
  3. Asian Journal of Pharmaceutical Sciences (2018) IF: 4.56
    Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice

    DOI: 10.1016/j.ajps.2018.04.001

    PMID: 99999999

    Sample: Serum
  4. Immunity & Ageing (2019) IF: 4.019
    Decreased Immunoglobulin G in Brain Regions of Elder Female APOE4-TR Mice Accompany With Aβ Accumulation

    DOI: 10.1186/s12979-018-0142-7

    PMID: 30700991

    Sample: Plasma,Tissue homogenate
  5. Scientific Reports (2016) IF: 4.259
    Protein Kinase D2 Protects Against Acute Colitis Induced By Dextran Sulfate Sodium In Mice.

    DOI: 10.1038/srep34079

    PMID: 27659202

    Sample: Serum
  6. Research in veterinary science (2019) IF: 1.892
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue homogenate
  7. Molecular medicine reports (2019) IF: 1.922
    Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

    DOI: 10.3892/mmr.2019.9868

    PMID: 30664205

    Sample: Serum
  8. Functional Foods in Health and Disease (2018)
    Immunological effects of Kefir produced from Kefir grains versus starter cultures when fed to mice

    DOI: 10.31989/ffhd.v8i8.533

    PMID: 99999999

    Sample: Tissue homogenate
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