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Mouse IL-12(Interleukin 12) ELISA Kit

Citations(41)Uniprot : P43432

  • Cat.No.:E-EL-M3062

  • Reactivity: Mouse

To Purchase E-EL-M3062

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 pg/mL
Sensitivity 0.17 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12. You can calculate the concentration of Mouse IL-12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 0.94 2.48 6.82 0.91 2.37 6.40
Standard deviation 0.06 0.13 0.35 0.05 0.12 0.33
CV (%) 6.48 5.39 5.08 5.61 4.97 5.19

Recovery

The recovery of Mouse IL-12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 90
EDTA plasma (n=8) 88-100 95
Cell culture media (n=8) 95-110 102

Linearity

Samples were spiked with high concentrations of Mouse IL-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P43432
SynonymsIL12,IL-12 p70, IL12 p70
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. ADVANCED MATERIALS (2023) IF: 32.086
    Tumor Cell Nanovaccines based on Genetically Engineered Antibody-anchored Membrane

    DOI: 10.1002/adma.202208923

    PMID: 36715052

    		 Sample: bone-marrow-derived dendritic cells (BMDCs)
  2. ADVANCED FUNCTIONAL MATERIALS (2020) IF: 16.836
    Quantitative Photoacoustic Diagnosis and Precise Treatment of Inflammation In Vivo Using Activatable Theranostic Nanoprobe

    DOI: 10.1002/adfm.202001771
    Sample: Serum
  3. Journal for ImmunoTherapy of Cancer (2023) IF: 12.469
    Novel combination strategy of high intensity focused ultrasound (HIFU) and checkpoint blockade boosted by bioinspired and oxygen-supplied nanoprobe for multimodal imaging-guided cancer therapy

    DOI: 10.1136/jitc-2022-006226
    Sample: Serum
  4. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    Biomimetic nanoprobe-augmented triple therapy with photothermal, sonodynamic and checkpoint blockade inhibits tumor growth and metastasis

    DOI: 10.1186/s12951-022-01287-y

    PMID: 35168608

    		 Sample: tumor cell
  5. Cell Reports (2021) IF: 9.423
    Expression of miRNA-29 in Pancreatic β Cells Promotes Inflammation and Diabetes via TRAF3

    DOI: 10.1016/j.celrep.2020.108576

    PMID: 33406428

    		 Sample: Cell sample
  6. Cell Reports (2023) IF: 9.9949
    RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.

    DOI: 10.1016/j.celrep.2023.112097
    Sample: serum,RAW264.7 cell
  7. Oxidative Medicine and Cellular Longevity (2023) IF: 7.31
    Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function

    DOI: 10.1155/2023/2894695
    Sample: colonic tissue
  8. Frontiers in Microbiology (2022) IF: 6.064
    Recombinant Listeria ivanovii strain expressing listeriolysin O in place of ivanolysin O might be a potential antigen carrier for vaccine construction

    DOI: 10.3389/fmicb.2022.962326

    PMID: 35935244

    		 Sample: splenocytes
  9. International Journal of Nanomedicine (2021) IF: 6.4
    Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation

    DOI: 10.2147/IJN.S318363
    Sample: serum,CD8+ T cell,BMDCs
  10. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Norcantharidin liposome emulsion hybrid delivery system enhances PD-1/PD-L1 immunotherapy by agonizing the non-canonical NF-κB pathway

    DOI: 10.1016/j.ijpharm.2022.122361
  11. Biomaterials Science (2021) IF: 6.843
    Resolving the hepatic fibrosis via suppressing oxidative stress and inflammatory response by novel hyaluronic acid modified nanocomplex

    DOI: 10.1039/D1BM01499D
    Sample: serum
  12. Oncotarget (2016) IF: 5.008
    Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

    DOI: 10.18632/oncotarget.12958
    Sample: serum,lymphocytes
  13. Oncotarget (2017) IF: 5.168
    λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine

    DOI: 10.18632/oncotarget.15610
    Sample: Dendritic cell
  14. Frontiers in Chemistry (2022) IF: 5.545
    Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

    DOI: 10.3389/fchem.2022.955995

    PMID: 36034656

    		 Sample: 4T1 cell
  15. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    		 Sample: Splenocyte
  16. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

    DOI: 10.3390/ijms23010226

    PMID: 35008651

    		 Sample: serum,Splenocyte
  17. Foods (2020) IF: 4.092
    Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis

    DOI: 10.3390/foods10010058

    PMID: 33383792

    		 Sample: colon tissue,serum
  18. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    		 Sample: Cell culture supernatant
  19. Frontiers in Microbiology (2020) IF: 4.236
    Toxoplasma gondii ROP16I Deletion: The Exacerbated Impact on Adverse Pregnant Outcomes in Mice

    DOI: 10.3389/fmicb.2019.03151
    Sample: placental tissue,serum,spleen cell
  20. Scientific Reports (2017) IF: 4.259
    Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine

    DOI: 10.1038/srep43796
    Sample: Cell culture supernatant
  21. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte‐endothelial interactions and protects against acute graft‐versus‐host disease

    DOI: 10.1111/jcmm.15434
    Sample: Plasma
  22. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell culture supernatant
  23. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929
    Sample: Serum,Cell lysate,Tissue homogenate
  24. Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
    Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

    DOI: 10.1155/2019/1475729

    PMID: 31531179

    		 Sample: Tissue homogenate
  25. Food & Function (2017) IF: 3.247
    Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae

    DOI: 10.1039/C7FO00227K
    Sample: Cell culture supernatant
  26. PHARMACOLOGY (2023) IF: 3.429
    DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

    DOI: 10.1159/000528634
    Sample: serum，nasal lavage fluid
  27. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    		 Sample: Serum,Tissue homogenate
  28. Journal of Functional Foods (2018) IF: 3.47
    Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model

    DOI: 10.1016/j.jff.2018.07.001
    Sample: Tissue homogenate
  29. ACS Omega (2022) IF: 3.512
    Macrophage Distribution Affected by Virus-Encoded Granulocyte Macrophage Colony Stimulating Factor Combined with Lactate Oxidase

    DOI: 10.1021/acsomega.2c03213

    PMID: 35847295

    		 Sample: RAW264.7 cell
  30. RESPIRATORY RESEARCH (2020) IF: 3.924
    Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma

    DOI: 10.1186/s12931-020-01587-7
    Sample: RAW264.7 cell
  31. Scientific Reports (2020) IF: 3.998
    Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway

    DOI: 10.1038/s41598-020-66789-x

    PMID: 32555320

    		 Sample: Serum
  32. PARASITOLOGY RESEARCH (2016) IF: 2.027
    spp. in experimentally infected immunocompetent and immunosuppresed mice

    DOI: 10.1007/s00436-016-4951-3
    Sample: Serum,Tissue homogenate
  33. PeerJ (2020) IF: 2.379
    The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis

    DOI: 10.7717/peerj.8294

    PMID: 32030319

    		 Sample: Tissue homogenate
  34. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 2.559
    Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway

    DOI: 10.1016/j.bbrc.2018.12.010
    Sample: Tissue homogenate,Cell culture supernatant
  35. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway

    DOI: 10.1155/2022/7421265

    PMID: 35178108

    		 Sample: serum
  36. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6.

    DOI: 10.1155/2022/9092311
    Sample: nasal mucosa tissue
  37. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    		 Sample: Serum,Cell culture supernatant
  38. Molecular Medicine Reports (2019) IF: 2.952
    BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

    DOI: 10.3892/mmr.2019.10780
    Sample: BMDCs
  39. JOURNAL OF FOOD BIOCHEMISTRY (2019) IF: 1.358
    Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress

    DOI: 10.1111/jfbc.13050

    PMID: 31571245

    		 Sample: Serum
  40. AESTHETIC PLASTIC SURGERY (2018) IF: 1.484
    Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8
    Sample: Cell culture supernatant
  41. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2019)
    ROS Generated by Upconversion Nanoparticle-Mediated Photodynamic Therapy Induces Autophagy via PI3K/AKT/ mTOR Signaling Pathway in M1 Peritoneal Macrophage.

    DOI: 10.33594/000000093
    Sample: peritoneal macrophages
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