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Mouse IL-13(Interleukin 13) ELISA Kit

Citations(31)Uniprot : P20109

Cat.No.:E-EL-M0727
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IL-13 in samples. No significant cross-reactivity or interference between Mouse IL-13 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-13. You can calculate the concentration of Mouse IL-13 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-13 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	100.90	188.89	944.30	108.13	197.24	874.46
Standard deviation	6.85	8.46	30.69	7.27	10.06	28.33
CV (%)	6.79	4.48	3.25	6.72	5.10	3.24

Recovery

The recovery of Mouse IL-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-106	97
EDTA plasma (n=8)	88-101	95
Cell culture media (n=8)	91-105	98

Linearity

Samples were spiked with high concentrations of Mouse IL-13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P20109
Synonyms	IL13, ALRH, BHR1, P600
Research Area	Cancer, Cardiovascular, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Death Discovery (2022) IF: 5.241

Immunofluorescence Troubleshooting Tips

DOI: 10.1038/s41420-022-00960-3
PMID: 35383145	Sample: Serum

Clinical and Translational Allergy (2022) IF: 5.871

MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

DOI: 10.1002/clt2.12114
PMID: 35079347	Sample: Bronchoalveolar Lavage Fluid (Balf),Lung Tissue

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924

Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

DOI: 10.3390/ijms23010226
PMID: 35008651	Sample: Splenocyte

AEROBIOLOGIA (2018) IF: 1.515

Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

DOI: 10.1007/s10453-018-9533-7
	Sample: Plasma

RESEARCH IN VETERINARY SCIENCE (2019) IF: 1.751

Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

DOI: 10.1016/j.rvsc.2019.08.012
PMID: 31412308	Sample: Serum,Tissue Homogenate

PARASITOLOGY INTERNATIONAL (2016) IF: 2.23

Effect of Corilagin on the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model

DOI: 10.1016/j.parint.2016.03.001
	Sample: Serum

Experimental and Therapeutic Medicine (2020) IF: 2.447

Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate‑induced colitis

DOI: 10.3892/etm.2020.9138
	Sample: Serum

BIOLOGICAL RESEARCH (2019) IF: 2.482

Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

DOI: 10.1186/s40659-019-0216-9
PMID: 30823938	Sample: Plasma

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 2.559

Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway

DOI: 10.1016/j.bbrc.2018.12.010
	Sample: Tissue Homogenate,Cell Culture Supernatant

MICROBIAL PATHOGENESIS (2019) IF: 2.581

Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

DOI: 10.1016/j.micpath.2019.103557
	Sample: Small Intestinal Tissues

EUROPEAN JOURNAL OF PHARMACOLOGY (2016) IF: 2.73

Mechanism of Corilagin interference with IL-13/STAT6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model

DOI: 10.1016/j.ejphar.2016.11.018
	Sample: Serum

Journal of Immunology Research (2019) IF: 3.404

C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice

DOI: 10.1155/2019/5148575
PMID: 31886304	Sample: Serum

Frontiers in Pharmacology (2019) IF: 3.845

Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion

DOI: 10.3389/fphar.2019.01422
	Sample: Serum,Tissue Homogenate

INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943

Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma

DOI: 10.1016/j.intimp.2020.107223
	Sample: Bronchoalveolar Lavage Fluid

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192

Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

DOI: 10.1021/acs.jafc.0c00461
PMID: 32182051	Sample: Serum,Cell Culture Supernatant

Scientific Reports (2017) IF: 4.259

Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice

DOI: 10.1038/srep42655
	Sample: Bronchoalveolar Lavage Fluid,Serum

Scientific Reports (2022) IF: 4.38

7-Methoxyisoflavone ameliorates atopic dermatitis symptoms by regulating multiple signaling pathways and reducing chemokine production

DOI: 10.1038/s41598-022-12695-3
PMID: 35610286	Sample: Dorsal Skin,Ears

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486

Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein

DOI: 10.1111/jcmm.15485
	Sample: Tissue Homogenate

ACS Chemical Neuroscience (2020) IF: 4.486

Autophagy Activation Represses Pyroptosis through the IL-13 and JAK1/STAT1 Pathways in a Mouse Model of Moderate Traumatic Brain Injury

DOI: 10.1021/acschemneuro.0c00517
PMID: 33170612	Sample: Plasma

Nutrients (2020) IF: 4.546

Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

DOI: 10.3390/nu12010249
PMID: 31963703	Sample: Serum,Cell Culture Supernatant

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872

Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway

DOI: 10.1016/j.ecoenv.2020.110491
PMID: 32213367	Sample: Cell Culture Supernatant

INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932

Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

DOI: 10.1016/j.intimp.2021.108266
PMID: 34678694	Sample: Balf

CHEMOSPHERE (2019) IF: 5.108

Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring

DOI: 10.1016/j.chemosphere.2019.125356
PMID: 31743867	Sample: Tissue Homogenate

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279

Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation

DOI: 10.1021/acs.jafc.1c00559
PMID: 33974424	Sample: Cell Culture Supernatant

FREE RADICAL BIOLOGY AND MEDICINE (2019) IF: 5.657

Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis

DOI: 10.1016/j.freeradbiomed.2019.09.009
	Sample: Bronchoalveolar Lavage Fluid,Tissue Homogenate

Nutrients (2021) IF: 5.719

Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

DOI: 10.3390/nu13093209
PMID: 34579088	Sample: Splenocyte

Food & Function (2022) IF: 6.317

Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways

DOI: 10.1039/D2FO00777K
PMID: 35920097	Sample: Serum

IMMUNOLOGY (2021) IF: 7.397

PTBP1 is necessary for dendritic cells to regulate T-cell homeostasis and antitumour immunity

DOI: 10.1111/imm.13304
PMID: 33421118	Sample: Bronchoalveolar Lavage Fluid

FOOD CHEMISTRY (2021) IF: 7.514

A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

DOI: 10.1016/j.foodchem.2021.131589
	Sample: Spleen Cells

ENVIRONMENTAL POLLUTION (2022) IF: 9.988

Juvenile arsenic exposure aggravates goblet cell hyperplasia and airway mucus secretion in ovalbumin-sensitized mice

DOI: 10.1016/j.envpol.2022.120462
PMID: 36270563	Sample: Lung Tissue

SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753

Aerofloral studies and allergenicity of dominant pollen types in Taraba and Bauchi States of Northeastern Nigeria

DOI: 10.1016/j.scitotenv.2022.157076
PMID: 35780899	Sample: Plasma

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Reviews/Q&A

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Reviews
Q&A

M***********dSubmitted [ Aug 09 2023 ]

Asked: Can this kit be used for BALF (bronchoalveolar lavagae fluid) supernatant? Also what dilution can be used for that sample?

adminSubmitted [ Aug 09 2023 ]

Answered: This kit can be used for BALF supernatant sample, and it is recommended to test the sample without dilution.

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