×

Mouse IL-13(Interleukin 13) ELISA Kit

  • Cat.No.:E-EL-M0727

  • Reactivity: Mouse

To Purchase E-EL-M0727

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-13 in samples. No significant cross-reactivity or interference between Mouse IL-13 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-13. You can calculate the concentration of Mouse IL-13 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-13 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 100.90 188.89 944.30 108.13 197.24 874.46
Standard deviation 6.85 8.46 30.69 7.27 10.06 28.33
CV (%) 6.79 4.48 3.25 6.72 5.10 3.24

Recovery

The recovery of Mouse IL-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-106 97
EDTA plasma (n=8) 88-101 95
Cell culture media (n=8) 91-105 98

Linearity

Samples were spiked with high concentrations of Mouse IL-13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P20109
SynonymsIL13, ALRH, BHR1, P600
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Free Radical Biology and Medicine (2019) IF: 6.17
    Oral administration of 3,3'-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis

    DOI: 10.1016/j.freeradbiomed.2019.09.009

    PMID: 31521664

    Sample: Bronchoalveolar Lavage Fluid,Tissue homogenate
  2. Chemosphere (2019) IF: 5.108
    Maternal 1-nitropyrene exposure during pregnancy increases susceptibility of allergic asthma in adolescent offspring

    DOI: 10.1016/j.chemosphere.2019.125356

    PMID: 31743867

    Sample: Tissue homogenate
  3. Journal of Cellular and Molecular Medicine (2020) IF: 4.486
    Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes-associated protein

    DOI: 10.1111/jcmm.15485

    PMID: 32542982

    Sample: Tissue homogenate
  4. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    Sample: Serum,Cell culture supernatant
  5. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    Sample: Serum,Cell culture supernatant
  6. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway

    DOI: 10.1016/j.ecoenv.2020.110491

    PMID: 32213367

    Sample: Cell culture supernatant
  7. Scientific Reports (2017) IF: 4.259
    Rheb1 Deletion In Myeloid Cells Aggravates OVA-Induced Allergic Inflammation In Mice.

    DOI: 10.1038/srep42655

    PMID: 28225024

    Sample: Bronchoalveolar Lavage Fluid,Serum
  8. Journal of immunology research (2019) IF: 3.327
    C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice

    DOI: 10.1155/2019/5148575

    PMID: 31886304

    Sample: Serum
  9. Frontiers in Pharmacology (2019) IF: 3.845
    Ameliorative Effects of Suhuang Antitussive Capsule on LPS-Induced Sputum Obstruction in Mice through Promoting HGF Secretion via an Intestinal Endocrine-Dependent Pathway

    DOI: 10.3389/fphar.2019.01422

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  10. Biochemical and biophysical research communications (2019) IF: 2.559
    Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IkB/P38 MAPK signaling pathway

    DOI: 10.1016/j.bbrc.2018.12.010

    PMID: 30528734

    Sample: Tissue homogenate,Cell culture supernatant
  11. Biological research (2019) IF: 2.357
    Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

    DOI: 10.1186/s40659-019-0216-9

    PMID: 30823938

    Sample: Plasma
  12. European Journal of Pharmacology (2016) IF: 2.896
    Mechanism Of Corilagin Interference With IL-13/STAT6 Signaling Pathways In Hepatic Alternative Activation Macrophages In Schistosomiasis-Induced Liver Fibrosis In Mouse Model.

    DOI: 10.1016/j.ejphar.2016.11.018

    PMID: 27845069

    Sample: Serum
  13. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate-induced colitis

    DOI: 10.3892/etm.2020.9138

    PMID: 32934675

    Sample: Serum
  14. Research in veterinary science (2019) IF: 1.892
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue homogenate
  15. Aerobiologia (2019) IF: 1.515
    Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

    DOI: 10.1007/s10453-018-9533-7

    PMID: 99999999

    Sample: Plasma
  16. Parasitology International (2016) IF: 1.744
    Effect Of Corilagin On The miR-21/smad7/ERK Signaling Pathway In A Schistosomiasis-Induced Hepatic Fibrosis Mouse Model.

    DOI: 10.1016/j.parint.2016.03.001

    PMID: 26946098

    Sample: Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha: