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Mouse IL-18(Interleukin 18) ELISA Kit

  • Cat.No.:E-EL-M0730

  • Reactivity: Mouse

To Purchase E-EL-M0730

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. You can calculate the concentration of Mouse IL-18 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-18 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.50 145.20 342.80 53.00 144.60 363.40
Standard deviation 2.60 8.10 16.80 3.70 7.40 12.40
CV (%) 5.25 5.58 4.90 6.98 5.12 3.41

Recovery

The recovery of Mouse IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma (n=8) 86-100 91
Cell culture media (n=8) 92-104 99

Linearity

Samples were spiked with high concentrations of Mouse IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P70380
SynonymsIL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell Death & Disease (2020) IF: 6.304
    Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype

    DOI: 10.1038/s41419-020-2565-2

    PMID: 32415059

    Sample: Tissue homogenate
  2. Biochimica Et Biophysica Acta-Molecular Basis of Disease (2017) IF: 5.476
    A Novel Mechanism Of Diabetic Vascular Endothelial Dysfunction: Hypoadiponectinemia-Induced NLRP3 Inflammasome Activation.

    DOI: 10.1016/j.bbadis.2017.02.012

    PMID: 28216285

    Sample: Serum,Cell culture supernatant
  3. Aging (2020) IF: 4.831
    Lipopolysaccharide upregulates miR-132/212 in Hirschsprung-associated enterocolitis, facilitating pyroptosis by activating NLRP3 inflammasome via targeting Sirtuin 1 (SIRT1)

    DOI: 10.18632/aging.103852

    PMID: 32950974

    Sample: Serum
  4. Aging (2020) IF: 4.831
    Salidroside ameliorates Parkinson's disease by inhibiting NLRP3-dependent pyroptosis

    DOI: 10.18632/aging.103215

    PMID: 32432571

    Sample: Cell culture supernatant,Tissue homogenate
  5. Frontiers in Cellular and Infection Microbiology (2020) IF: 4.123
    Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways

    DOI: 10.3389/fcimb.2020.00196

    PMID: 32432055

    Sample: Cell culture supernatant
  6. International Immunopharmacology (2020) IF: 3.943
    COX-2-PGE 2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

    DOI: 10.1016/j.intimp.2020.106801

    PMID: 32702600

    Sample: Tissue homogenate
  7. Frontiers in endocrinology (2018) IF: 3.519
    Verapamil ameliorates hepatic metaflammation by inhibiting thioredoxin-interacting protein/NLRP3 inflammasome activation

    DOI: 10.3389/fendo.2018.00640

    PMID: 30429827

    Sample: Serum
  8. Biomedicine & pharmacotherapy (2018) IF: 3.457
    Jinxin oral liquid inhibits human respiratory syncytial virus-induced excessive inflammation associated with blockade of the NLRP3/ASC/Caspase-1 pathway.

    DOI: 10.1016/j.biopha.2018.04.174

    PMID: 29864921

    Sample: Serum,Tissue homogenate
  9. Cytokine (2020) IF: 2.952
    6-Gingerol attenuates macrophages pyroptosis via the inhibition of MAPK signaling pathways and predicts a good prognosis in sepsis

    DOI: 10.1016/j.cyto.2019.154854

    PMID: 31539844

    Sample: Cell culture supernatant
  10. Inflammation (2019) IF: 2.884
    Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling

    DOI: 10.1007/s10753-019-00986-3

    PMID: 30847744

    Sample: Serum,Cell culture supernatant
  11. European Journal of Pharmacology (2016) IF: 2.896
    Liraglutide Attenuates Lipopolysaccharide-Induced Acute Lung Injury In Mice.

    DOI: 10.1016/j.ejphar.2016.10.016

    PMID: 27756605

    Sample: Bronchoalveolar Lavage Fluid
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