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Mouse IL-2(Interleukin 2) ELISA Kit

  • Cat.No.:E-EL-M0042

  • Reactivity: Mouse

To Purchase E-EL-M0042

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-2 in samples. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.00 87.40 385.60 52.40 82.20 365.00
Standard deviation 3.30 3.80 20.40 3.10 4.80 15.70
CV (%) 6.73 4.35 5.29 5.92 5.84 4.30

Recovery

The recovery of Mouse IL-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 89-102 96

Linearity

Samples were spiked with high concentrations of Mouse IL-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P04351
SynonymsIL2, TCGF, Lymphokine
Research AreaCancer,Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Advanced materials (2019) IF: 27.398
    Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy

    DOI: 10.1002/adma.201905751

    PMID: 31709671

    Sample: Tissue homogenate
  2. Scientific Reports (2017) IF: 4.259
    Rheb1 Deletion In Myeloid Cells Aggravates OVA-Induced Allergic Inflammation In Mice.

    DOI: 10.1038/srep42655

    PMID: 28225024

    Sample: Bronchoalveolar Lavage Fluid,Serum
  3. Carbohydrate Polymers (2016) IF: 4.811
    Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis.

    DOI: 10.1016/j.carbpol.2015.12.050

    PMID: 26876821

    Sample: Cell culture supernatant
  4. IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo
    Sample: Serum
  5. International Immunopharmacology (2019) IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo

    DOI: 10.1016/j.intimp.2019.05.047

    PMID: 31220695

    Sample: Serum
  6. Journal of Functional Foods (2017) IF: 3.144
    Antitumor And Immunomodulatory Effects Of Ginsenoside Rh2 And Its Octyl Ester Derivative In H22 Tumor-Bearing Mice.

    DOI: 10.1016/j.jff.2017.03.013

    Sample: Serum
  7. Microbial Pathogenesis (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    Sample: Serum,Cell culture supernatant
  8. Microbial Pathogenesis (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

    DOI: 10.1016/j.micpath.2020.104054

    PMID: 32061917

    Sample: Serum
  9. Gene (2016) IF: 2.415
    Immunogenicity Of The Recombinant HxuCBA Proteins Encoded By HxuCBA Gene Cluster Of Haemophilus Parasuis In Mice.

    DOI: 10.1016/j.gene.2016.07.001

    PMID: 27378742

    Sample: Serum
  10. Journal of Virological Methods (2020) IF: 1.786
    Colonisation of mice and pigs by a chimeric porcine circovirus 1-2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines

    DOI: 10.1016/j.jviromet.2020.113905

    PMID: 32502500

    Sample: Cell culture supernatant
  11. Research in veterinary science (2019) IF: 1.892
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue homogenate
  12. International Journal of Clinical and Experimental Medicine (2015) IF: 1.069
    Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.

    PMID: 26884954

    Sample: Cell culture supernatant
  13. Plant Archives (2020)
    IMPACT OF BACTERIAL LIPOPOLYSACCHARIDE ON CYTOKINES RESPONSES AT PREGNANCY

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
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