Click here to view cell apoptosis assay products

Mouse IL-5(Interleukin 5) ELISA Kit

  • Cat.No.:E-EL-M0722

  • Reactivity: Mouse

To Purchase E-EL-M0722

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-5 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-5. You can calculate the concentration of Mouse IL-5 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-5 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-5 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.34 86.56 464.22 51.71 92.29 432.78
Standard deviation 2.80 5.01 24.37 3.55 4.08 15.41
CV (%) 5.56 5.79 5.25 6.87 4.42 3.56


The recovery of Mouse IL-5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 96
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 87-102 94


Samples were spiked with high concentrations of Mouse IL-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P04401
SynonymsIL5, EDF, TRF, E-CSF, ESGF
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    Aerofloral studies and allergenicity of dominant pollen types in Taraba and Bauchi States of Northeastern Nigeria

    DOI: 10.1016/j.scitotenv.2022.157076

    PMID: 35780899

    Sample: plasma
  2. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589

    Sample: Spleen cells
  3. Food & Function (2022) IF: 6.317
    Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways

    DOI: 10.1039/D2FO00777K

    PMID: 35920097

    Sample: serum
  4. Cell Death Discovery (2022) IF: 5.241
    IL-37 isoform D acts as an inhibitor of soluble ST2 to boost type 2 immune homeostasis in white adipose tissue

    DOI: 10.1038/s41420-022-00960-3

    PMID: 35383145

    Sample: Serum
  5. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    Sample: Splenocyte
  6. Clinical and Translational Allergy (2022) IF: 5.871
    MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

    DOI: 10.1002/clt2.12114

    PMID: 35079347

    Sample: bronchoalveolar lavage fluid (BALF),Lung tissue
    Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

    DOI: 10.3390/ijms23010226

    PMID: 35008651

    Sample: Splenocyte
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    Sample: Serum,Cell culture supernatant
    In vitro and in vivo relaxation and anti-inflammation of natural flavonoids from Elaeagnus pungens leaf via L-type calcium channel and targeting MAPK signal pathway

    DOI: 10.1016/j.jep.2021.114071

    PMID: 33831464

    Sample: Cell culture supernatant
    Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein

    DOI: 10.1111/jcmm.15485

    Sample: Tissue homogenate
  11. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    Sample: Serum,Cell culture supernatant
    Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

    DOI: 10.1016/j.intimp.2021.108266

    PMID: 34678694

    Sample: BALF
  13. INFLAMMATION (2020) IF: 3.212
    Casticin Attenuates Osteoarthritis-Related Cartilage Degeneration by Inhibiting the ROS-Mediated NF-κB Signaling Pathway in vitro and in vivo

    DOI: 10.1007/s10753-019-01167-y

    Sample: serum,ADTC5 cell
  14. PHARMACOLOGY (2023) IF: 3.429
    DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

    DOI: 10.1159/000528634

    Sample: serum,nasal lavage fluid
    Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma

    DOI: 10.1016/j.intimp.2020.107223

    Sample: Bronchoalveolar Lavage Fluid
  16. Dose-Response (2021) IF: 2.658
    Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice:

    DOI: 10.1177/15593258211016257

    Sample: Plasma
  17. Molecular Medicine Reports (2019) IF: 2.952
    BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

    DOI: 10.3892/mmr.2019.10780

    Sample: BMDCs
  18. AEROBIOLOGIA (2018) IF: 1.515
    Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

    DOI: 10.1007/s10453-018-9533-7

    Sample: Plasma
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?