Mouse LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit (E-EL-M3114)

Name: Mouse LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit
Brand: Elabscience
SKU: E-EL-M3114
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Mouse LAB7-1 (B-Lymphocyte Activation Antigen B7-1) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes LAB7-1 in samples.No significant cross-reactivity or interference between LAB7-1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	2.275	2.319	2.297	2.210
10	1.534	1.542	1.538	1.451
5	0.886	0.884	0.885	0.798
2.5	0.553	0.549	0.551	0.464
1.25	0.293	0.293	0.293	0.206
0.63	0.192	0.186	0.189	0.102
0.31	0.145	0.135	0.140	0.053
0	0.084	0.090	0.087	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.030	2.170	8.070	0.970	2.180	7.850
Standard deviation	0.060	0.120	0.510	0.080	0.190	0.530
CV（%）	5.720	5.740	6.280	7.910	8.820	6.700

Recovery

The recovery of Mouse LAB7-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	92-106	99
Serum （n=8）	85-98	92
EDTA plasma （n=8）	89-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse LAB7-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-109	98-114	96-110
1:2	Average	100	106	102
1:4	Range	89-105	96-113	96-109
1:4	Average	96	104	102
1:8	Range	88-101	99-114	95-111
1:8	Average	95	105	102

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	97.47-99.06
Sample 2（n=16）	93.28-101.81

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LAB7-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LAB7-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LAB7-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LAB7-1 . You can calculate the concentration of LAB7-1 in the samples by comparing the OD of the samples to the standard curve.

Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1,B7,and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages,and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells,macrophages,and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation,proliferation,and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152,also known as CTLA-4,to deliver an inhibitory signal to T cells.