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Mouse LH(Luteinizing Hormone) ELISA Kit

Citations(40)

  • Cat.No.:E-EL-M3053

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse LH in samples. No significant cross-reactivity or interference between Mouse LH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse LH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LH. You can calculate the concentration of Mouse LH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 12 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 12 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse LH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse LH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.81 2.24 8.84 0.89 2.37 8.19
Standard deviation 0.04 0.11 0.41 0.05 0.12 0.38
CV (%) 4.94 4.91 4.64 5.62 5.06 4.64

Recovery

The recovery of Mouse LH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-105 101
EDTA plasma (n=8) 88-100 93
Cell culture media (n=8) 88-96 93

Linearity

Samples were spiked with high concentrations of Mouse LH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsLutropin, Lutrophin
Research AreaNeuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Endocrinology (2022) IF: 6.055
    Role of EGFR expressed on the granulosa cells in the pathogenesis of polycystic ovarian syndrome.

    DOI: 10.3389/fendo.2022.971564
    Sample: Serum
  2. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway

    DOI: 10.1016/j.bioorg.2020.104295
    Sample: Serum
  3. Food Science and Human Wellness (2022) IF: 5.154
    The protective effects of Procyanidin C-1 on bisphenol a-induced testicular dysfunction in aged mice

    DOI: 10.1016/j.fshw.2022.03.020
    Sample: Serum
  4. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2021) IF: 0.533
    Effect of total flavonoids of Cuscuta chinensis Lam. (Convolvulaceae) on oxidative stress injury in mouse testis and epididymis, and on serum levels of reproductive hormones in oligoasthenospermia mice model

    DOI: 10.4314/tjpr.v18i6.15
    Sample: Serum,Tissue Homogenate
  5. REPRODUCTION FERTILITY AND DEVELOPMENT (2020) IF: 1.721
    High-fat diet leads to elevated lipid accumulation and endoplasmic reticulum stress in oocytes, causing poor embryo development

    DOI: 10.1071/RD20112

    PMID: 32998795

    		 Sample: Blood
  6. DRUG AND CHEMICAL TOXICOLOGY (2019) IF: 1.946
    Fluted pumpkin seeds protect against busulfan-induced oxidative stress and testicular injuries in adult mice

    DOI: 10.1080/01480545.2019.1657885

    PMID: 31469002

    		 Sample: Plasma
  7. REPRODUCTION FERTILITY AND DEVELOPMENT (2022) IF: 1.973
    Anti-tuberculosis drugs used in a directly observed treatment short course (DOTS) schedule alter endocrine patterns and reduce the ovarian reserve and oocyte quality in the mouse

    DOI: 10.1071/RD22108

    PMID: 36219878

    		 Sample: Serum
  8. Reproductive Biology (2022) IF: 2.376
    The effect of icariin on autoimmune premature ovarian insufficiency via modulation of Nrf2/HO-1/Sirt1 pathway in mice

    DOI: 10.1016/j.repbio.2022.100638

    PMID: 35344846

    		 Sample: Serum
  9. MOLECULAR REPRODUCTION AND DEVELOPMENT (2020) IF: 2.609
    Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency

    DOI: 10.1002/mrd.23404
    Sample: Serum
  10. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

    DOI: 10.1155/2021/9968463

    PMID: 34987599

    		 Sample: Serum
  11. Reproductive Biology and Endocrinology (2018) IF: 2.852
    The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement

    DOI: 10.1186/s12958-018-0365-7
    Sample: Serum
  12. Chinese Medicine (2020) IF: 2.96
    Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice

    DOI: 10.1186/s13020-020-00405-y

    PMID: 33292347

    		 Sample: Serum
  13. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
    Rescue of obesity-induced infertility in female mice by silencing AgRP neurons

    DOI: 10.1016/j.bbrc.2022.07.032

    PMID: 35870259

    		 Sample: Blood
  14. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322
    Prepubertal exposure to copper oxide nanoparticles induces Leydig cell injury with steroidogenesis disorders in mouse testes

    DOI: 10.1016/j.bbrc.2023.02.067
    Sample: Serum
  15. Heliyon (2020) IF: 3.776
    The intake of an extract from seeds of Tamarindus indica L. modulates the endocrine function of adult male mice under a high fat diet

    DOI: 10.1016/j.heliyon.2020.e03310

    PMID: 32051875

    		 Sample: Serum
  16. REPRODUCTIVE BIOMEDICINE ONLINE (2021) IF: 3.828
    Ovarian inflammatory mRNA profiles of a dehydroepiandrosterone plus high-fat diet-induced polycystic ovary syndrome mouse model

    DOI: 10.1016/j.rbmo.2021.10.024
    Sample: Serum
  17. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2020) IF: 3.871
    MiR-199-3p modulates the onset of puberty in rodents probably by regulating the expression of Kiss1 via the p38 MAPK pathway

    DOI: 10.1016/j.mce.2020.110994
    Sample: Serum
  18. Scientific Reports (2019) IF: 4.011
    Gestational exposure to an epidemiologically defined mixture of phthalates leads to gonadal dysfunction in mouse offspring of both sexes

    DOI: 10.1038/s41598-019-42377-6
    Sample: Plasma
  19. Genes (2021) IF: 4.096
    DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis

    DOI: 10.3390/genes12111800

    PMID: 34828406

    		 Sample: Plasma
  20. TOXICOLOGY (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    		 Sample: Serum
  21. Scientific Reports (2017) IF: 4.259
    Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

    DOI: 10.1038/srep43475
    Sample: Serum
  22. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model

    DOI: 10.1093/biolre/ioac004
    Sample: Serum
  23. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice

    DOI: 10.1016/j.jep.2021.113996

    PMID: 33684516

    		 Sample: Serum
  24. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2022) IF: 4.369
    CXCL13 and CXCR5 are upregulated in PCOS mice ovaries but downregulated following metformin administration

    DOI: 10.1016/j.mce.2022.111730

    PMID: 35921919

    		 Sample: Serum
  25. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-505-3p is a repressor of puberty onset in female mice

    DOI: 10.1530/JOE-18-0533
    Sample: Serum
  26. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-29 family regulates the puberty onset mediated by a novel Gnrh1 transcription factor TBX21

    DOI: 10.1530/JOE-19-0082
    Sample: Serum
  27. Scientific Reports (2022) IF: 4.996
    Effects of Cordyceps militaris fermentation products on reproductive development in juvenile male mice

    DOI: 10.1038/s41598-022-18066-2

    PMID: 35962055

    		 Sample: Serum
  28. ENDOCRINOLOGY (2022) IF: 5.051
    Distinctions in PCOS induced by letrozole versus dehydroepiandrosterone with high-fat diet in mouse model

    DOI: 10.1210/endocr/bqac097
    Sample: Serum
  29. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Irisin deletion induces a decrease in growth and fertility in mice

    DOI: 10.1186/s12958-021-00702-7

    PMID: 33581723

    		 Sample: Serum
  30. Food & Function (2022) IF: 5.396
    Tilapia skin peptides restore cyclophosphamide-induced premature ovarian failure via inhibiting oxidative stress and apoptosis in mice

    DOI: 10.1039/D1FO04239D

    PMID: 35083997

    		 Sample: Serum
  31. Communications Biology (2019) IF: 6.268
    In utero exposure to acetaminophen and ibuprofen leads to intergenerational accelerated reproductive aging in female mice

    DOI: 10.1038/s42003-019-0552-x
    Sample: Serum
  32. International Journal of Nanomedicine (2022) IF: 6.4
    Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway

    DOI: 10.2147/IJN.S348673

    PMID: 35401001

    		 Sample: Serum
  33. METABOLISM-CLINICAL AND EXPERIMENTAL (2019) IF: 6.513
    Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome

    DOI: 10.1016/j.metabol.2019.02.002

    PMID: 30768966

    		 Sample: Serum
  34. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway

    DOI: 10.1016/j.biopha.2022.113061

    PMID: 35658231

    		 Sample: Serum
  35. SCIENCE OF THE TOTAL ENVIRONMENT (2020) IF: 6.551
    Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice

    DOI: 10.1016/j.scitotenv.2020.141049
    Sample: Serum
  36. Frontiers in Immunology (2022) IF: 7.561
    TNFAIP3 Reduction-of-Function Drives Female Infertility and CNS Inflammation.

    DOI: 10.3389/fimmu.2022.811525
    Sample: Serum
  37. DIABETES (2020) IF: 7.72
    Ablation of AMPK-Related Kinase MPK38/MELK Leads to Male-Specific Obesity in Aged Mature Adult Mice.

    DOI: 10.2337/db20-0436
    Sample: Serum
  38. ENVIRONMENTAL POLLUTION (2021) IF: 8.071
    Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice

    DOI: 10.1016/j.envpol.2021.117557

    PMID: 34167001

    		 Sample: Serum
  39. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice

    DOI: 10.1016/j.freeradbiomed.2022.10.314

    PMID: 36309297

    		 Sample: Testicular Tissues
  40. Particle and Fibre Toxicology (2022) IF: 9.4
    Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway

    DOI: 10.1186/s12989-022-00453-2

    PMID: 35177090

    		 Sample: Serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

S***********sSubmitted [ Jul 06 2023 ]

Asked: Has this ELISA been tested using urine?

Reply

A

adminSubmitted [ Jul 06 2023 ]

Answered: According to our internal tests, the E-EL-M3053 kit can detect urine samples.

Q

S***********sSubmitted [ Jul 06 2023 ]

Asked: Has this ELISA been used to test the LH levels of pregnant mice at different E days?

Reply

A

adminSubmitted [ Jul 06 2023 ]

Answered: From the citations of E-EL-M3053, the detection report about LH levels of pregnant mice at different E days have not been found at present.

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