Mouse MMP-13(Matrix Metalloproteinase 13) ELISA Kit (E-EL-M0076)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-13. You can calculate the concentration of Mouse MMP-13 in the samples by comparing the OD of the samples to the standard curve.

Mouse adrenocorticotropic hormone (ACTH), also called corticotropin, is a 39-amino-acid polypeptide derived by proteolytic processing of pro-opiomelanocortin (POMC). It is produced mainly by corticotroph cells of the anterior pituitary, with smaller amounts generated in the hypothalamus, skin, and immune cells. Secretion is stimulated by hypothalamic corticotropin-releasing hormone and vasopressin, follows a pronounced circadian rhythm, and is suppressed by glucocorticoid negative feedback. ACTH acts principally through the melanocortin 2 receptor (MC2R) on adrenal cortical cells to stimulate glucocorticoid synthesis and secretion and to maintain adrenal cortical growth and steroidogenic capacity. Receptor binding activates the cyclic AMP-protein kinase A pathway and upregulates steroidogenic enzymes and cholesterol mobilization, positioning ACTH as the central effector of the hypothalamic-pituitary-adrenal axis during stress. Additional melanocortin receptors mediate effects on pigmentation and lipid metabolism. Circulating ACTH is used to evaluate integrity of the hypothalamic-pituitary-adrenal axis, to distinguish primary from secondary adrenal insufficiency, and to investigate Cushing disease; paired measurement with cortisol helps localize the lesion. This ELISA kit enables quantitative determination of mouse ACTH in serum, plasma, and cell culture supernatant, supporting dynamic endocrine testing, stress neuroendocrine research, and studies of pituitary-adrenal regulation.