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Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

Citations(26)Uniprot : P29477

  • Cat.No.:E-EL-M0696

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 2.90 9.90 1.00 3.00 10.50
Standard deviation 0.10 0.20 0.30 0.10 0.20 0.50
CV (%) 9.09 6.90 3.03 10.00 6.67 4.76

Recovery

The recovery of Mouse NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 96
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 95-109 101

Linearity

Samples were spiked with high concentrations of Mouse NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P29477
SynonymsNOS2A, HEP-NOS, I-NOS
Research AreaCancer, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Cell Reports (2023) IF: 9.9949
    RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.

    DOI: 10.1016/j.celrep.2023.112097
    Sample: RAW264.7 cell
  2. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023) IF: 8.025
    Folic acid-modified lactoferrin nanoparticles coated with a laminarin layer loaded curcumin with dual-targeting for ulcerative colitis treatment

    DOI: 10.1016/j.ijbiomac.2023.123229
    Sample: Colonic tissue
  3. Theranostics (2019) IF: 8.0630
    Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

    DOI: 10.7150/thno.31461
    Sample: mammary gland tumor tissue
  4. JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
    Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

    DOI: 10.1016/j.jid.2021.04.019

    PMID: 34029575

    		 Sample: Serum,Tissue homogenate
  5. BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
    Phillygenin inhibited M1 macrophage polarization and reduced hepatic stellate cell activation by inhibiting macrophage exosomal miR-125b-5p

    DOI: 10.1016/j.biopha.2023.114264
    Sample: RAW264.7 cell
  6. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Enhancement of the functionality of attenuating acute lung injury by a microemulsion formulation with volatile oil of Angelicae Sinensis Radix and Ligusticum Chuanxiong Rhizoma encapsulated

    DOI: 10.1016/j.biopha.2022.113888

    PMID: 36272263

    		 Sample: serum
  7. GLIA (2021) IF: 7.452
    Astrocytic VEGFA: An essential mediator in blood–brain-barrier disruption in Parkinson's disease

    DOI: 10.1002/glia.24109
    Sample: primary astrocytes,pericytes
  8. Journal of Materials Chemistry B (2022) IF: 7.571
    Mesoporous hollow Fe3O4 nanoparticles regulate the behavior of neuro-associated cells through induction of macrophages polarization in an alternating magnetic field

    DOI: 10.1039/D2TB00527A
    Sample: RAw 264.7 cell
  9. FOOD CHEMISTRY (2020) IF: 6.306
    Genipin-crosslinked human serum albumin coating using a tannic acid layer for enhanced oral administration of curcumin in the treatment of ulcerative colitis

    DOI: 10.1016/j.foodchem.2020.127241

    PMID: 32540526

    		 Sample: Tissue homogenate
  10. Nanomedicine-Nanotechnology Biology and Medicine (2021) IF: 6.458
    pH/ROS dual-sensitive and chondroitin sulfate wrapped poly (β-amino ester)-SA-PAPE copolymer nanoparticles for macrophage-targeted oral therapy for ulcerative colitis

    DOI: 10.1016/j.nano.2021.102461

    PMID: 34562656

    		 Sample: colon tissue
  11. BIOMACROMOLECULES (2021) IF: 6.988
    Entrapment of Macrophage-Target Nanoparticles by Yeast Microparticles for Rhein Delivery in Ulcerative Colitis Treatment

    DOI: 10.1021/acs.biomac.1c00425

    PMID: 34019390

    		 Sample: Tissue homogenate
  12. CELLULAR & MOLECULAR BIOLOGY LETTERS (2018) IF: 5.787
    Inhibition of inflammation using diacerein markedly improved renal function in endotoxemic acute kidney injured mice

    DOI: 10.1186/s11658-018-0107-z

    PMID: 30140293

    		 Sample: Tissue homogenate
  13. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2021) IF: 5.875
    Sequential administration of sialic acid-modified liposomes as carriers for epirubicin and zoledronate elicit stronger antitumor effects with reduced toxicity

    DOI: 10.1016/j.ijpharm.2021.120552

    PMID: 33798685

    		 Sample: Tissue Slice
  14. Frontiers in Bioengineering and Biotechnology (2021) IF: 5.89
    Lentinan-Based Oral Nanoparticle Loaded Budesonide With Macrophage-Targeting Ability for Treatment of Ulcerative Colitis

    DOI: 10.3389/fbioe.2021.702173
    Sample: colon tissue
  15. Journal of Functional Foods (2021) IF: 4.451
    Structural characterization and immunomodulatory activity of a glucan from Radix Codonopsis

    DOI: 10.1016/j.jff.2021.104537
    Sample: Cell culture supernatant
  16. MOLECULAR PHARMACEUTICS (2018) IF: 4.556
    Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection

    DOI: 10.1021/acs.molpharmaceut.8b00781

    PMID: 30234992

    		 Sample: Plasma,Tissue homogenate
  17. VETERINARY MICROBIOLOGY (2022) IF: 3.293
    IL-5 enhances the resistance of Actinobacillus pleuropneumoniae infection in mice through maintaining appropriate levels of lung M2, PMN-II and highly effective neutrophil extracellular traps

    DOI: 10.1016/j.vetmic.2022.109438

    PMID: 35468400

    		 Sample: Bronchoalveolar lavage fluid (BALF)
  18. TOXICOLOGY IN VITRO (2021) IF: 3.5
    Triclosan affects the expression of nitric oxide synthases (NOSs), peroxisome proliferator-activated receptor gamma (PPARγ), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in mouse neocortical neurons in vitro

    DOI: 10.1016/j.tiv.2021.105143

    PMID: 33722737

    		 Sample: Cell culture supernatant
  19. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021) IF: 3.575
    Arbutin protects brain against middle cerebral artery occlusion-reperfusion (MCAo/R) injury

    DOI: 10.1016/j.bbrc.2021.09.006

    PMID: 34507065

    		 Sample: brain
  20. NEUROCHEMICAL RESEARCH (2021) IF: 3.996
    Platinum nanoparticles Protect Against Lipopolysaccharide-Induced Inflammation in Microglial BV-2 Cells via Decreased Oxidative Damage and Increased Phagocytosis

    DOI: 10.1007/s11064-021-03434-7

    PMID: 34432181

    		 Sample: BV-2 cell
  21. Molecular Medicine Reports (2020) IF: 2.1
    Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway

    DOI: 10.3892/mmr.2020.11455

    PMID: 33000274

    		 Sample: Cell culture supernatant
  22. Biomed Research International (2018) IF: 2.583
    Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response

    DOI: 10.1155/2018/7239181
    Sample: BV2 cells
  23. Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
    Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)

    DOI: 10.22038/IJBMS.2021.59348.13174
    Sample: RAW 264.7 cell
  24. NEUROCHEMICAL RESEARCH (2019) IF: 2.782
    The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

    DOI: 10.1007/s11064-019-02746-z

    PMID: 30759294

    		 Sample: Cell lysate
  25. AESTHETIC PLASTIC SURGERY (2018) IF: 1.484
    Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8
    Sample: Cell culture supernatant
  26. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2019)
    ROS Generated by Upconversion Nanoparticle-Mediated Photodynamic Therapy Induces Autophagy via PI3K/AKT/ mTOR Signaling Pathway in M1 Peritoneal Macrophage.

    DOI: 10.33594/000000093
    Sample: peritoneal macrophages
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