Current Position:Home>>Kits>>ELISA Kits>> Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit
Cat.No.:E-EL-M0696
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.10 | 2.90 | 9.90 | 1.00 | 3.00 | 10.50 |
Standard deviation | 0.10 | 0.20 | 0.30 | 0.10 | 0.20 | 0.50 |
CV (%) | 9.09 | 6.90 | 3.03 | 10.00 | 6.67 | 4.76 |
The recovery of Mouse NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-103 | 96 |
EDTA plasma (n=8) | 89-102 | 95 |
Cell culture media (n=8) | 95-109 | 101 |
Samples were spiked with high concentrations of Mouse NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P29477
|
Synonyms | NOS2A, HEP-NOS, I-NOS |
Research Area | Cancer, Metabolism, Neuroscience |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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Sample: RAW264.7 cell |
Sample: Colonic tissue |
DOI: 10.7150/thno.31461 |
|
Sample: mammary gland tumor tissue |
PMID: 34029575 |
Sample: Serum,Tissue homogenate |
Sample: RAW264.7 cell |
PMID: 36272263 |
Sample: serum |
DOI: 10.1002/glia.24109 |
|
Sample: primary astrocytes,pericytes |
DOI: 10.1039/D2TB00527A |
|
Sample: RAw 264.7 cell |
PMID: 32540526 |
Sample: Tissue homogenate |
PMID: 34562656 |
Sample: colon tissue |
PMID: 34019390 |
Sample: Tissue homogenate |
PMID: 30140293 |
Sample: Tissue homogenate |
PMID: 33798685 |
Sample: Tissue Slice |
Sample: colon tissue |
Sample: Cell culture supernatant |
PMID: 30234992 |
Sample: Plasma,Tissue homogenate |
PMID: 35468400 |
Sample: Bronchoalveolar lavage fluid (BALF) |
PMID: 33722737 |
Sample: Cell culture supernatant |
PMID: 34507065 |
Sample: brain |
PMID: 34432181 |
Sample: BV-2 cell |
PMID: 33000274 |
Sample: Cell culture supernatant |
DOI: 10.1155/2018/7239181 |
|
Sample: BV2 cells |
Sample: RAW 264.7 cell |
PMID: 30759294 |
Sample: Cell lysate |
Sample: Cell culture supernatant |
DOI: 10.33594/000000093 |
|
Sample: peritoneal macrophages |