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Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

  • Cat.No.:E-EL-M0696

  • Reactivity: Mouse

To Purchase E-EL-M0696

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 2.90 9.90 1.00 3.00 10.50
Standard deviation 0.10 0.20 0.30 0.10 0.20 0.50
CV (%) 9.09 6.90 3.03 10.00 6.67 4.76


The recovery of Mouse NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-103 96
EDTA plasma (n=8) 89-102 95
Cell culture media (n=8) 95-109 101


Samples were spiked with high concentrations of Mouse NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P29477
Research AreaCancer, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Carbohydrate Polymers (2021) IF: 9.381
    Calcium pectinate and hyaluronic acid modified lactoferrin nanoparticles loaded rhein with dual-targeting for ulcerative colitis treatment

    DOI: 10.1016/j.carbpol.2021.117998

    PMID: 33858583

    Sample: Tissue homogenate
  2. Food Chemistry (2021) IF: 7.514
    Genipin-crosslinked human serum albumin coating using a tannic acid layer for enhanced oral administration of curcumin in the treatment of ulcerative colitis

    DOI: 10.1016/j.foodchem.2020.127241

    PMID: 32540526

    Sample: Tissue homogenate
  3. Molecular pharmaceutics (2018) IF: 4.556
    Combination therapy with DETA/NO and clopidogrel inhibits metastasis in murine mammary gland cancer models via improved vasoprotection

    DOI: 10.1021/acs.molpharmaceut.8b00781

    PMID: 30234992

    Sample: Plasma,Tissue homogenate
  4. Molecular Medicine Reports (2020) IF: 2.1
    Osthole inhibits proliferation of kainic acid-activated BV-2 cells by modulating the Notch signaling pathway

    DOI: 10.3892/mmr.2020.11455

    PMID: 33000274

    Sample: Cell culture supernatant
  5. Neurochemical research (2019) IF: 2.772
    The VGVAPG Peptide Regulates the Production of Nitric Oxide Synthases and Reactive Oxygen Species in Mouse Astrocyte Cells In Vitro

    DOI: 10.1007/s11064-019-02746-z

    PMID: 30759294

    Sample: Cell lysate
  6. Cellular & molecular biology letters (2018) IF: 1.291
    Inhibition of inflammation using diacerein markedly improved renal function in endotoxemic acute kidney injured mice

    DOI: 10.1186/s11658-018-0107-z

    PMID: 30140293

    Sample: Tissue homogenate
  7. Aesthetic plastic surgery (2019) IF: 1.484
    M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8

    PMID: 30552469

    Sample: Cell culture supernatant
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