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Mouse SELP(P-Selectin) ELISA Kit

  • Cat.No.:E-EL-M0638

  • Reactivity: Mouse

To Purchase E-EL-M0638

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse SELP in samples. No significant cross-reactivity or interference between Mouse SELP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse SELP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. You can calculate the concentration of Mouse SELP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse SELP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse SELP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.30 4.30 0.50 1.20 4.20
Standard deviation 0.03 0.07 0.18 0.03 0.06 0.16
CV (%) 6.00 5.38 4.19 6.00 5.00 3.81

Recovery

The recovery of Mouse SELP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-109 99
EDTA plasma (n=8) 90-104 95
Cell culture media (n=8) 92-103 98

Linearity

Samples were spiked with high concentrations of Mouse SELP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q01102
SynonymsCD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice

    DOI: 10.1155/2020/8430465

    PMID: 32377309

    Sample: Serum
  2. Molecular pharmaceutics (2018) IF: 4.556
    Combination therapy with DETA/NO and clopidogrel inhibits metastasis in murine mammary gland cancer models via improved vasoprotection

    DOI: 10.1021/acs.molpharmaceut.8b00781

    PMID: 30234992

    Sample: Plasma,Tissue homogenate
  3. American Journal of Physiology-Renal Physiology (2018) IF: 3.164
    ADAMTS13 protects mice against renal ischemia-reperfusion injury by reducing inflammation and improving endothelial function

    DOI: 10.1152/ajprenal.00405.2018

    PMID: 30461292

    Sample: Plasma,Tissue homogenate
  4. Journal of cardiovascular pharmacology (2018) IF: 2.277
    NaoXinTong Capsule Inhibits Carrageenan-Induced Thrombosis in Mice.

    DOI: 10.1097/FJC.9999999900000592

    PMID: 29738376

    Sample: Serum
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