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Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit

Cat.No.:E-EL-M1040
Reactivity: Mouse

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse sIgA in samples. No significant cross-reactivity or interference between Mouse sIgA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse sIgA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sIgA. You can calculate the concentration of Mouse sIgA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse sIgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse sIgA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.51	1.17	4.34	0.46	1.16	4.60
Standard deviation	0.03	0.06	0.24	0.03	0.06	0.18
CV (%)	5.88	5.13	5.53	6.52	5.17	3.91

Recovery

The recovery of Mouse sIgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	95
EDTA plasma (n=8)	88-101	94
Cell culture media (n=8)	87-103	94

Linearity

Samples were spiked with high concentrations of Mouse sIgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Microbiology (2022) IF: 6.064
Early life administration of Bifidobacterium bifidum BD-1 alleviates long-term colitis by remodeling the gut microbiota and promoting intestinal barrier development

DOI: 10.3389/fmicb.2022.916824

PMID: 35935215
Sample: cecal contents
Nutrients (2022) IF: 6.706
Preventive Mechanism of Lycopene on Intestinal Toxicity Caused by Cyclophosphamide Chemotherapy in Mice by Regulating TLR4-MyD88/TRIF-TRAF6 Signaling Pathway and Gut-Liver Axis

DOI: 10.3390/nu14214467

PMID: 36364730
Sample: Intestinal Mucosa
Journal of Functional Foods (2022) IF: 4.451
Anti-allergic effects of two potential probiotic strains isolated from infant feces in China

DOI: 10.1016/j.jff.2022.105070

Sample: Cecal contents
Asian Journal of Pharmaceutical Sciences (2018) IF: 4.56
Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice

DOI: 10.1016/j.ajps.2018.04.001

Sample: Serum
Journal of Immunology Research (2022) IF: 4.818
Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen

DOI: 10.1155/2022/7200379

PMID: 35465352
Sample: Intestinal segments
Toxins (2020) IF: 3.531
Betulinic Acid Ameliorates the T-2 Toxin-Triggered Intestinal Impairment in Mice by Inhibiting Inflammation and Mucosal Barrier Dysfunction through the NF-κB Signaling Pathway

DOI: 10.3390/toxins12120794

PMID: 33322178
Sample: Serum
Food & Nutrition Research (2021) IF: 3.894
Effects of different doses of omega-3 polyunsaturated fatty acids on gut microbiota and immunity

DOI: 10.29219/fnr.v65.6263

PMID: 34349613
Sample: serum
PARASITOLOGY RESEARCH (2016) IF: 2.027
spp. in experimentally infected immunocompetent and immunosuppresed mice

DOI: 10.1007/s00436-016-4951-3

Sample: Serum,Tissue homogenate
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition

DOI: 10.1155/2022/5394514

PMID: 35178105
Sample: plasma
Indian Journal of Medical Microbiology (2021) IF: 0.985
Generating Mucosal and Systemic Immune Response Following Vaccination of Vibrio cholerae Adhesion Molecule against Shigella flexneri Infection

DOI: 10.4103/ijmm.IJMM_19_411

Sample: ileum mucous

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