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Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit

  • Cat.No.:E-EL-M1040

  • Reactivity: Mouse

To Purchase E-EL-M1040

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse sIgA in samples. No significant cross-reactivity or interference between Mouse sIgA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse sIgA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sIgA. You can calculate the concentration of Mouse sIgA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse sIgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse sIgA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.51 1.17 4.34 0.46 1.16 4.60
Standard deviation 0.03 0.06 0.24 0.03 0.06 0.18
CV (%) 5.88 5.13 5.53 6.52 5.17 3.91


The recovery of Mouse sIgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma (n=8) 88-101 94
Cell culture media (n=8) 87-103 94


Samples were spiked with high concentrations of Mouse sIgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Frontiers in Microbiology (2022) IF: 6.064
    Early life administration of Bifidobacterium bifidum BD-1 alleviates long-term colitis by remodeling the gut microbiota and promoting intestinal barrier development

    DOI: 10.3389/fmicb.2022.916824

    PMID: 35935215

    Sample: cecal contents
  2. Nutrients (2022) IF: 6.706
    Preventive Mechanism of Lycopene on Intestinal Toxicity Caused by Cyclophosphamide Chemotherapy in Mice by Regulating TLR4-MyD88/TRIF-TRAF6 Signaling Pathway and Gut-Liver Axis

    DOI: 10.3390/nu14214467

    PMID: 36364730

    Sample: Intestinal Mucosa
  3. Journal of Functional Foods (2022) IF: 4.451
    Anti-allergic effects of two potential probiotic strains isolated from infant feces in China

    DOI: 10.1016/j.jff.2022.105070

    Sample: Cecal contents
  4. Asian Journal of Pharmaceutical Sciences (2018) IF: 4.56
    Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice

    DOI: 10.1016/j.ajps.2018.04.001

    Sample: Serum
  5. Journal of Immunology Research (2022) IF: 4.818
    Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen

    DOI: 10.1155/2022/7200379

    PMID: 35465352

    Sample: Intestinal segments
  6. Toxins (2020) IF: 3.531
    Betulinic Acid Ameliorates the T-2 Toxin-Triggered Intestinal Impairment in Mice by Inhibiting Inflammation and Mucosal Barrier Dysfunction through the NF-κB Signaling Pathway

    DOI: 10.3390/toxins12120794

    PMID: 33322178

    Sample: Serum
  7. Food & Nutrition Research (2021) IF: 3.894
    Effects of different doses of omega-3 polyunsaturated fatty acids on gut microbiota and immunity

    DOI: 10.29219/fnr.v65.6263

    PMID: 34349613

    Sample: serum
    spp. in experimentally infected immunocompetent and immunosuppresed mice

    DOI: 10.1007/s00436-016-4951-3

    Sample: Serum,Tissue homogenate
  9. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition

    DOI: 10.1155/2022/5394514

    PMID: 35178105

    Sample: plasma
  10. Indian Journal of Medical Microbiology (2021) IF: 0.985
    Generating Mucosal and Systemic Immune Response Following Vaccination of Vibrio cholerae Adhesion Molecule against Shigella flexneri Infection

    DOI: 10.4103/ijmm.IJMM_19_411

    Sample: ileum mucous
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