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Mouse SST(Somatostatin) ELISA Kit

Citations(4)Uniprot : P60041

Cat.No.:E-EL-M1086
Reactivity: Mouse

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T
Assay time	2.0h
Detection range	7.81-500 pg/mL
Sensitivity	4.69 pg/mL
Sample type &Sample volume	Serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of SST concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SST. During the reaction, SST in the sample or standard competes with a fixed amount of SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SST. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SST in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SST were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SST were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	26.50	74.00	196.80	28.70	68.50	190.70
Standard deviation	1.40	4.10	10.20	1.50	2.70	8.40
CV (%)	5.28	5.54	5.18	5.23	3.94	4.40

Recovery

The recovery of SST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-103	95
EDTA plasma(n=8)	84-97	90
Cell culture media(n=8)	86-96	91

Target Information

Database Links	SwissProt: P60041
Synonyms	GHIH, SRIF, SMST, GHRIH, GHRIF, Growth Hormone Releasing Inhibiting Hormone, Somatotropin Release-Inhibiting Factor, Somatostatin-14, Somatostatin-28

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

CELL RESEARCH (2021) IF: 25.617
Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans

DOI: 10.1038/s41422-021-00486-w

PMID: 33692492
Sample: Serum
Nutrients (2022) IF: 6.706
Prevention of Loperamide-Induced Constipation in Mice and Alteration of 5-Hydroxytryotamine Signaling by Ligilactobacillus salivarius Li01

DOI: 10.3390/nu14194083

PMID: 36235735
Sample: serum
Frontiers in Pharmacology (2022) IF: 5.988
Codonopsis pilosula Polysaccharide Improved Spleen Deficiency in Mice by Modulating Gut Microbiota and Energy Related Metabolisms

DOI: 10.3389/fphar.2022.862763

PMID: 35559259
Sample: serum
Evidence-based Complementary and Alternative Medicine (2020) IF: 1.813
Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate-Induced Constipation in Mice

DOI: 10.1155/2020/1471824

PMID: 32148532
Sample: Serum

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