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Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit

  • Cat.No.:E-EL-M1153

  • Reactivity: Mouse

To Purchase E-EL-M1153

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.25-80 ng/mL
Sensitivity 0.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse TSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TSH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.40 7.30 36.90 4.40 7.10 39.00
Standard deviation 0.30 0.30 1.70 0.30 0.40 1.20
CV (%) 6.82 4.11 4.61 6.82 5.63 3.08


The recovery of Mouse TSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-98 93
EDTA plasma (n=8) 95-107 100
Cell culture media (n=8) 91-106 99


Samples were spiked with high concentrations of Mouse TSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Small Methods (2017) IF: 14.188
    Transdermal Delivery of Anti‐Obesity Compounds to Subcutaneous Adipose Tissue with Polymeric Microneedle Patches

    DOI: 10.1002/smtd.201700269

    Sample: Serum
    Repositioning of Cefuroxime as novel selective inhibitor of the thyroid hormone activating enzyme type 2 Deiodinase

    DOI: 10.1016/j.phrs.2023.106685

    Sample: Blood
    Transcriptome Profile of Thyroid Glands in Bile Duct Ligation Mouse Model

    DOI: 10.3390/ijms23158244

    PMID: 35897811

    Sample: serum
    Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway

    DOI: 10.1016/j.ecoenv.2019.02.024

    PMID: 30771657

    Sample: Plasma
  5. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells

    DOI: 10.1155/2021/2482453

    PMID: 34804362

    Sample: plasma
  6. THYROID (2020) IF: 5.309
    The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation

    DOI: 10.1089/thy.2019.0557

    PMID: 32111151

    Sample: Cell culture supernatant
  7. Journal of Immunology Research (2022) IF: 4.493
    Saikosaponin-d Attenuates Hashimoto’s Thyroiditis by Regulating Macrophage Polarization

    DOI: 10.1155/2022/7455494

    Sample: blood
    Renal protective effects of grape seed extract treatment against Eltroxin-induced hyperthyroidism, kidney damage, and oxidative stress in male mice

    DOI: 10.1007/s11356-020-08210-8

    Sample: serum
  9. Reproductive Biology and Endocrinology (2019) IF: 2.589
    Reduced expression of PD-L1 in autoimmune thyroiditis attenuate trophoblast invasion through ERK/MMP pathway

    DOI: 10.1186/s12958-019-0536-1

    PMID: 31656199

    Sample: Tissue homogenate
    Subclinical hypothyroidism contributes to poor glycemic control in patients with type 2 diabetes mellitus, and ellagic acid attenuates methimazole-induced abnormal glucose metabolism in mice model

    DOI: 10.1111/jfbc.13753

    PMID: 33955004

    Sample: Serum
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