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Cat.No.:E-MSEL-M0024
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Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 1.56-100 pg/mL |
Sensitivity | 0.94 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse C5a in samples. No significant cross-reactivity or interference between Mouse C5a and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse C5a concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse C5a were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse C5a were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 4.68 | 15.48 | 37.24 | 4.84 | 14.93 | 38.16 |
Standard deviation | 0.33 | 0.82 | 1.37 | 0.31 | 0.79 | 1.41 |
CV (%) | 6.98 | 5.31 | 3.67 | 6.38 | 5.30 | 3.70 |
The recovery of Mouse C5a spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-104 | 96 |
EDTA plasma (n=8) | 85-101 | 92 |
Cell culture media (n=8) | 86-100 | 92 |
Samples were spiked with high concentrations of Mouse C5a and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P06684
|
Synonyms | C5a |
Research Area | Cell Biology, Metabolism, Immunology, Signal transduction |
PMID: 34655413 |
Sample: serum |
PMID: 31901551 |
Sample: Cell culture supernatant |