Current Position:Home>>Kits>>ELISA Kits>> MS Mouse GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit
Cat.No.:E-MSEL-M0022
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Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.67 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse GM-CSF concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GM-CSF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GM-CSF were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 24.96 | 70.05 | 170.32 | 24.85 | 66.63 | 155.77 |
Standard deviation | 1.30 | 3.34 | 7.78 | 1.69 | 3.57 | 6.85 |
CV (%) | 5.19 | 4.77 | 4.57 | 6.79 | 5.36 | 4.40 |
The recovery of Mouse GM-CSF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 87-102 | 93 |
EDTA plasma (n=8) | 88-103 | 94 |
Cell culture media (n=8) | 86-101 | 92 |
Samples were spiked with high concentrations of Mouse GM-CSF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01587
|
Synonyms | GMCSF, CSF2 |
Research Area | Cancer, Immunology |
PMID: 34820587 |
Sample: B16–F10 cells,Red blood cells |
PMID: 35860242 |
Sample: serum |
PMID: 34295211 |
Sample: serum |
Sample: serum,RAW264.7 cell |
PMID: 33639205 |
Sample: Serum,Tissue(spleen) |
Sample: Cell culture supernatant,Tissue homogenate,serum |
PMID: 33973702 |
Sample: Serum |