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MS Mouse GROα/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

Citations(6)Uniprot : P12850

Cat.No.:E-MSEL-M0026
Reactivity: Mouse

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MS Series

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	48T/96T/96
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	10.01 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 25μL
Specificity	This kit recognizes Mouse GROα/CXCL1 in samples. No significant cross-reactivity or interference between Mouse GROα/CXCL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse GROα/CXCL1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROα/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GROα/CXCL1. You can calculate the concentration of Mouse GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GROα/CXCL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GROα/CXCL1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	49.96	139.61	359.77	47.33	130.77	359.19
Standard deviation	2.86	7.86	17.34	2.62	5.30	12.72
CV (%)	5.72	5.63	4.82	5.53	4.05	3.54

Recovery

The recovery of Mouse GROα/CXCL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-108	100
EDTA plasma (n=8)	85-98	92
Cell culture media (n=8)	94-106	101

Linearity

Samples were spiked with high concentrations of Mouse GROα/CXCL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P12850
Synonyms	NAP3, GRO1, GRO-A, MGSA, MGSA-A, SCYB1, FSP, CINC-1
Research Area	Cancer, Immunology

Assay Procedures

Citations

CELL RESEARCH (2015) IF: 12.413
P2RX 7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy

DOI: 10.1038/cr.2015.61

Sample: Serum
Redox Biology (2022) IF: 10.787
CXCL1-CXCR2 signalling mediates hypertensive retinopathy by inducing macrophage infiltration

DOI: 10.1016/j.redox.2022.102438

PMID: 35981418
Sample: serum
Oncotarget (2017) IF: 5.168
Wu-tou decoction attenuates neuropathic pain via suppressing spinal astrocytic IL-1R1/TRAF6/JNK signaling

DOI: 10.18632/oncotarget.21638

Sample: Tissue homogenate
FASEB JOURNAL (2020) IF: 4.966
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

DOI: 10.1096/fj.201902910RRR

Sample: Cell culture supernatant,Tissue homogenate,serum
ACTA PHARMACOLOGICA SINICA (2017) IF: 3.223
Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis

DOI: 10.1038/aps.2017.110

Sample: Tissue homogenate
ANDROLOGIA (2022) IF: 2.775
IL-17 exacerbates experimental autoimmune prostatitis via CXCL1/CXCL2-mediated neutrophil infiltration

DOI: 10.1111/and.14455

PMID: 35560069
Sample: prostate tissue

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