Current Position:Home>>Kits>>ELISA Kits>> MS Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit
Cat.No.:E-MSEL-M0041
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Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 p40 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 27.02 | 60.23 | 190.57 | 29.48 | 61.46 | 176.59 |
Standard deviation | 1.80 | 3.23 | 7.58 | 1.96 | 2.85 | 5.40 |
CV (%) | 6.65 | 5.37 | 3.98 | 6.66 | 4.63 | 3.06 |
The recovery of Mouse IL-12 p40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-104 | 95 |
EDTA plasma (n=8) | 94-110 | 100 |
Cell culture media (n=8) | 95-108 | 100 |
Samples were spiked with high concentrations of Mouse IL-12 p40 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P43432
|
Synonyms | IL12B, IL-12B, CLMF2, NKSF2, p40, IL-12/IL-23 p40 |
Research Area | Cancer, Immunology |
PMID: 35399111 |
Sample: serum |
DOI: 10.3390/md20030197 |
|
PMID: 35323496 |
Sample: Dendritic cells (DCs) |
DOI: 10.3390/biom10010159 |
|
PMID: 31963790 |
Sample: Cell culture supernatant,Cell sample |
Sample: Cell sample,Serum |
Sample: serum |
PMID: 35041513 |
Sample: Dendritic cell(DC cell) |
Sample: Plasma |
Sample: Cell culture supernatant |